EVALUATION OF IN-VITRO EFFICACY OF THREE TYPES OF DISINFECTANTS AGAINST BIOFILM FORMATION ON DIFFERENT POULTRY CONTACT SURFACES

Document Type : Research article

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Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt.

Abstract

Biofilms pose a significant challenge to treating poultry diseases and managing poultry flocks. This study assessed the effect of three disinfectants frequently used in the poultry sector on 14-day mature mono- and mixed-species biofilms of Staphylococcus aureus (S. aureus) and Salmonella typhimurium (S. typhimurium) on galvanized steel (GS) and plastic (PL) surfaces. The surface type influenced cellular density in mono-species and mixed-species biofilms (p <0.05). A significant positive correlation was observed between the average log in our verified data and the biofilm density measurements made with crystal violet on various surface coupons (r = 0.94, r = 0.91; r = 0.94, r = 0.81) for S. aureus and S. typhimurium, respectively. Our findings indicated that Oxy Clean® is the most effective disinfection on PL surfaces, showing a 5-log reduction at low concentration (0.5%) and short contact time (10 minutes) when applied with S. typhimurium or mixed strain biofilm. On GS surfaces, the biofilm of the mono-species S. aureus exhibits total resistance to all disinfectants tested, except for Oxy Clean®, which is effective after an extended contact period of 20 minutes, resulting in the complete elimination of the biofilm. Conversely, S. typhimurium exhibits a high sensitivity to all disinfectants employed. The only disinfectants capable of effectively penetrating the mixed biofilm are Oxy Clean® and VirKon S®, provided they are used for 20 minutes at a concentration of 1%. Compared to mono-species biofilms, both bacteria exhibited greater resistance to disinfectants in mixed-species biofilms.

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