CANINE OOCYTES' NUCLEAR COMPETENCY PROFILE BOTH WITH AND WITHOUT DIRECT CONTACT WITH BOVINE GRANULOSA CELL MONOLAYER

Document Type : Research article

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Department of Clinical Sciences, College of Veterinary Medicine, King Faisal University, AL-Ahsa, 31982, Saudi Arabia

Abstract

ABSTRACT
The current study examined how the maturation of canine oocytes was impacted via direct interaction between oocytes and bovine granulosa cell monolayers (BGML). At 38.5°C in 5% CO₂, 5% O₂, and 90% N₂ for 72 hours, canine cumulus-oocyte complexes (COCs) were cultivated in DMEM (control), oocytes cultured in BGML fitted into 24-well plates (direct contact), and oocytes cultured in BGML using 24-well plates with multicell culture with inserts (indirect contact). Oocytes matured over direct contact with BGML had a greater total meiotic resumption rate (50.0%) than those matured over indirect contact with BGML (31.0%) and control (12.4%) (P<0.05). Similarly, oocytes that matured over direct contact with BGML had a larger proportion of oocytes that advanced past the germinal vesicle (GV) to metaphase I (MI) stages (22.8%) than those that matured over indirect contact with BGML (11.9%) and control (2.1%) (P<0.05). When oocytes were developed directly in BGML (12.9%), the proportion of them that came to the metaphase II (MII) stage was higher (P<0.05) than when they were matured indirectly with BGML (4.5%). It was concluded that cultivation of canine oocytes complex with direct contact to BGML leads to meiotic restart and enhancement of maturation rate than indirect contact to BGML.
Key words: Bovine granulosa cell monolayers, Canine, Oocyte, In vitro maturation
 




 

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