Document Type : Research article
Authors
1
Department of Pharmacology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
2
Department of Cytology and Histology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.
3
Department of Biochemistry, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
4
Pharmacology department, Faculty of veterinary medicine, Beni-suef University, Beni-suef, Egypt
Abstract
Flumethrin, a synthetic pyrethroid insecticide, is used to manage a variety of insects, including mites, fleas, and ticks. This study was conducted to investigate the possible neuroprotective potential of Amphora coffaeformis and Propolis against Flumethrin-induced neurotoxicity in rat. Sixty male albino rats were allocated into six equal groups as follow: Group 1: (Control group), Group 2: was received Flumethrin (10 mg/kg b.w.), Group 3: was received Amphora coffeaformis (772 mg/kg b.w.), Group 4: was received Propolis (400 mg/kg b.w.), Group 5: was received Amphora coffeaformis (772 mg/ kg b.w.) followed by Flumethrin (10 mg/kg b.w.) one hour later, Group 6: was given Propolis (400 mg/kg b.w.) followed by Flumethrin (10 mg/kg b.w.) one hour later. The medications were given orally by an intragastric gavage once daily for thirty days. After one month, rats were sacrificed; serum and brain samples were collected for biochemical, molecular and histopathological analysis. The results revealed that Flumethrin exposure precipitated a cascade of oxidative stress, evidenced by elevated malondialdehyde (MDA) levels, diminished catalase (CAT) and glutathione (GSH) activities, and dysregulated Nrf-2, AChE, and TNFα expression. Conversely, Amphora coffaeformis and Propolis supplementation exerted a neuroprotective effect, mitigating MDA accumulation, augmenting CAT and GSH activities, and modulating TNFα, Nrf-2, and AChE expression. In conclusion, Amphora coffaeformis and Propolis may serve as natural neuroprotective agents, counteracting Flumethrin-induced neurotoxicity through their antioxidant and anti-inflammatory properties.
Keywords
Main Subjects
)
View on SCiNiTO