DETECTION OF GENETICALLY MODIFIED INGREDIENTS IN POULTRY MIXED DIETS IN NINEVEH PROVINCE BASED ON PCR TECHNIQUE

Document Type : Research article

Authors

1 Department of Veterinary Public Health, College of Veterinary Medicine, University of Mosul, Mosul, Iraq

2 Office of Veterinary, Ministry of Agriculture,

Abstract

Biotechnology was applied in the agricultural field to improve the growth of some plants and develop the herbicide tolerance , These derived crops especially soybean were used as genetically modified ingredients in some livestock feeding especially in poultry feed.The current study was aimed to evaluate the presence of transgenic ingredients in some poultry mixed diets in Nineveh province through the detection of genetically modified regulators including both 35S promoter and Nopaline synthase terminator as well as the detection of Roundup Ready soybean in poultry mixed diets.Results revealed that 62% of poultry mixed diets samples were positive to the presence of Roundup Ready soybean gene and 71% of them positive to the 35S promoter gene existance, while 83.3% of imported soybean meal used to prepare poultry mixed diets were positive to the existence of Roundup Ready soybean gene indicating the addition of transgenic ingredients in rations used to feed poultry in Nineveh province.The study showed that all local soybean meal examined are free from genetically modified regulatory elements.These results demonstrated the possibility of using polymerase chain reaction technique as sensitive ,reproducible and easy applicable to detect the genetic modification in poultry feeds.

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