THE IMPACT OF REPEATED ADMINISTRATION OF SERRATIOPEPTIDASE ON ANESTHESIA

Serratiopeptidase is a proteolytic enzyme originally derived from the bacterium Serratia species. It has been shown to possess a range of therapeutic effects, including anti-inflammatory, analgesic, and fibrinolytic activities. The objective of this study was to evaluate the effects of serratiopeptidase on the anesthetic response in mice, specifically focusing on the changes in oxygen saturation (SpO2), respiratory rate, and heart rate during anesthesia induction with xylazine and ketamine. Mice were administered serratiopeptidase (5, 10 or 20 mg/kg) orally for 15 days prior to anesthesia induction with xylazine and ketamine (10 and 150 mg/kg, respectively). Anesthetic parameters, including onset, duration, and recovery time, were recorded. Compared to the control group, the 5 and 10 mg/kg serratiopeptidase groups exhibited a significantly delayed onset and prolonged duration of anesthesia. Conversely, the 20 mg/kg group showed a shorter onset time, and a shorter duration of anesthesia compared to the 5 and 10 mg/kg groups. Recovery time was prolonged in the 20 mg/kg group. Compared to the control group, all serratiopeptidase-treated groups exhibited significantly higher SpO₂. Additionally, all treated groups showed a significant decrease in heart rate and a significant increase in respiratory rate. These findings suggest that serratiopeptidase exerts a limited dose-dependent effect on anesthetic response, likely through complex mechanisms involving neurotransmitter modulation and/or anti-inflammatory actions. Furthermore, serratiopeptidase may influence the physiological parameters associated with anesthesia in mice. Further research is warranted to elucidate the underlying mechanism and explore the potential clinical implications of these findings.