EFFECT OF DOPAMINE ON IN VITRO MATURATION, FERTILIZATION AND DEVELOPMENTAL COMPETENCE OF BOVINE OOCYTES

Document Type : Research article

Authors

1 Department of Theriogenology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 36615, Egypt.

2 Artificial Insemination and Embryo Transfer Department, Animal Reproduction Institute, Agriculture Research Center (ARC).

Abstract

Proteins and other nitrogenous substances, such as polyamines, catecholamines, and nitric oxide require amino acids for their synthesis. The purpose of this study was to investigate the effect of dopamine supplementation in IVM (in vitro maturation) medium on fertilization and developmental rates of bovine oocytes. Ovaries with apparently normal reproductive organs were collected from cattle within 30 minutes of slaughter. Cumulus oocyte complexes (COCs) were collected by aspirating medium sized ovarian follicles (4-8 mm). Good quality oocytes were selected, washed and incubated in TCM199 supplemented with dopamine. Different concentrations of dopamine (0, 5, 10, 20 and 40 ng) were added to the IVM media. Oocytes were incubated for 20:22 hours at 38.5 C◦ under 5% CO2 in air with 90% humidity. A non-significant increase (P>0.05) in maturation, fertilization and developmental rates of bovine oocytes was observed when the maturation medium was supplemented with all concentrations used (5, 10, 20, and 40) ng of dopamine. Dopamine non-significantly improves IVM, IVF (invitro fertilization) and developmental rates.

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