MOLECULAR DETECTION OF BOVINE VIRAL DIARRHEA VIRUS IN WHOLE BLOOD AND ORAL SWABS IN CATTLE AND BUFFALOES

Document Type : Research article

Authors

Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Assiut University, Postal code: 71526, Egypt

Abstract

Bovine viral diarrhea (BVD), a common viral disease, can affect large ruminants, both domesticated and wild. In Assiut Governorate, there is little information about BVD despite their financial losses. Thus, the aim of this investigation was to study the current situation of BVDV infection among large ruminants in Assiut Governorate. In this study, 39 cattle and 11 buffaloes were obtained from various villages in the Assiut Governorate, Egypt. Whole blood and oral lesions swabs were collected for laboratory investigation using RT-PCR. Nine cattle and one buffalo were found to have BVDV-RNA. The clinical findings accompanied by BVDV infection in examined animals were fully discussed. The species, age, and sex of the animals that underwent molecular testing had no significant effect on the BVDV infection rate (P<0.05). Meanwhile, Assiut Governorate's climatic conditions were found to have a significant influence (P<0.001) on the BVD infection rate, in which the prevalence of BVDV infection was highest in the summer season. Emphasizing the importance of putting effective prevention and control measures in place throughout Egypt is recommended in order to minimize the prevalence of BVDV.

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