MOLECULAR DETECTION OF HYALOMMA SPP. ISOLATED FROM SHEEP AND CAMEL IN THE AL-SAMAWAH DESERT OF AL-MUTHANNA PROVINCE, IRAQ

Document Type : Research article

Authors

1 Department of Parasitology, College of Veterinary Medicine, Al-Qasim Green University, 51013 Babylon, Iraq

2 Department of Microbiology, College of Veterinary Medicine, University of Al-Muthanna, Iraq

Abstract

Ticks are a common source for a variety of microorganisms especially those which are pathogenic or highly fatal. Hyalomma species are potential vectors for the CCHF virus. This study sought to identify Hyalomma spp. isolated form sheep and camel found in the Al-Samawah desert of Al-Muthanna Province. Sixty samples of ticks were used in this study, and each was subjected to PCR and partial gene sequencing using the 18S rRNA gene. The results from the analysis showed that 45 of 60 samples (75%) were identified as Hyalomma spp. While 17 PCR-purified products were chosen for sequencing, and the aligned data were used to build the phylogenetic tree. Uphill climbing the roof of phylogenetic analysis, the result reigned these isolates in this study as Hyalomma asiaticum related to several isolates in GenBank. This research has shown that these ticks of sheep and camel in the Al-Samawah desert of Al-Muthanna Province are commonly a marker of many viruses, including the CCHF virus. Further in-depth work ought to be completed as a priority for disease control purposes.

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Assiut Veterinary Medical Journal
Volume 70, Issue 183
October 2024
Pages 603-610

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