HISTOPATHOLOGICAL AND BIOCHEMICAL ASSESSMENT OF LIVER FIBROSIS INDUCED BY CARBON TETRACHLORIDE ADMINISTARTION IN RAT

Document Type : Research article

Authors

1 Animal Health Research Institute, Sohag, Egypt

2 Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Assiut University, Egypt.

3 Pathology and clinical Pathology. Animal Health Research Institute, Agriculture Research Center. Assiut, Egypt.

Abstract

This study investigated liver fibrosis and hepatotoxicity caused by carbon tetrachloride (CCL4) in albino rats. Two groups of twenty- two adult male rats each weighing 150 to 170 gms, were created. Ten rats from the CCL4-administered group (I) received subcutaneous injections of CCL4 in olive oil at a dose of 2 ml/kg twice a week for a total of 12 consecutive weeks. Twelve rats made up the control group (II), which was divided into two groups of six each. One group served as the standard control, and the other group only got olive oil via the same route and dosage as group (I). All rats were sacrificed 12 weeks post dosing and tissue specimens from livers were collected for histopathological examination. Additionally, serum samples were taken in order to measure various biochemical factors, including (alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein (TP), total antioxidant capacity (TAC) and lipid peroxidation (LPO). The histopathological examination of group I showed hepatic degenerative, necrotic and angiopathic alterations as well as pronounced hepatic fibrosis. The histopathological examination of group II showed normal hepatic appearance with no pathological changes. The biochemical results detected a significant upregulation in serum AST, ALT and LPO in group I compared to the control group while TP and TAC were significantly downregulated. In conclusion, the administered dose of CCL4 in rats caused variable degenerative and necrotic hepatic changes beside activation of the proliferative potential of collagen fibers and changed the biochemical parameters as compared to normal control rats.

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