Document Type : Research article
Authors
1
Biochemistry Dept., Animal Health Research Institute, Zagazig Branch, Agriculture Research Center (ARC), Egypt.
2
Biochemistry Department, Vet. Teaching Hospital Faculty of Vet. Med., Mosthor - Benha Univ, Egypt
3
Pharmacology Department, Vet. Teaching Hospital Faculty of Vet. Med., Mosthor - Benha Univ, Egypt
4
Clinical Pathology, Dept., Animal Health Research Institute, Zagazig Branch, Agriculture Research Center (ARC), Egypt.
Abstract
The present study was carried out to evaluate the effects of cisplatin on immunobiochemical parameters in rabbits and modulating by moringa leaves extract. A total of 20, healthy rabbits, 3-3.5 kg bwt were divided into 4 groups. Gp (1) control, Gp (2) received 5 mg/kg bwt IP cisplatin one dose every week for 4 weeks, Gp (3) received orally 200 mg of moringa olifera leaves extract/kg bwt daily for 4 weeks and Gp (4) received cisplatin and moringa leaves extract by same dose and periods. At 1st, and 10th days post administration 3 blood samples were taken from the ear vein for determination of some biochemical parameters. Cisplatin induced a significant decrease in WBCs, lymphocyte, phagocytic activity, killing %, total protein, albumin, total globulin, γ-globulin, HDL, GSH, SOD and CAT besides an insignificant decrease in basophil, α and β globulin coupled with a significant increase in AST, ALT ALP, urea, creatinine, cholesterol, triglycerides, LDL, VLDL, MDA and insignificant increase in neutrophil, monocyte and eosinophil at 1st-day post-administration. Moringa leaves extract induced a significant increase in WBCs, neutrophil, monocyte, phagocytic activity, killing %, total protein, albumin, total globulin, γ-globulin, HDL GSH SOD, CAT and a significant decrease in cholesterol, triglycerides, LDL, VLDL, MDA beside non significant decrease in lymphocyte beside insignificant increase in basophil, eosinophil, α, β globulin AST, ALT, ALP, creatinine, urea at 1st-day post-administration. Moringa leaves extract ameliorated the adverse effect of cisplatin by improving hematobiochemical and immuno-logical parameters and it returned to normal levels. It could be concluded that cisplatin induced adverse effects on biochemical parameters in rabbits. Using moringa leaves extract modulated the adverse effects of cisplatin. So, it is better to use antioxidants as moringa leaves extract with cisplatin
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