STUDIES ON MICROBIAL INFECTION ASSOCIATED REPEAT BREEDER IN BUFFALOES AND COWS AND ITS SENSITIVITY TO DIFFERENT ANTIBIOTICS IN VITRO

Document Type : Research article

Authors

1 Animal Health Research Institute EL-Minia Lab

2 Dept. of Theriogenology, Fac. of Vet. Med., Assiut University

Abstract

The present study aimed to throw light on the microbial infection and special mixed infection (bacterial and fungus) associated repeat breeder in buffaloes and cows. This work was carried out on 120 cervico-vaginal and uterine swabs collected from (50 buffaloes and 70 cows) suffering from repeat breeding (25 subclinically and 95 clinically infected cows from dairy farms at EL-Minia and Assiut Provinces. Bacteriological examination revealed that, 200 different microbial causative agents (30 isolates with incidence of 15% for subclinically and 170 isolates with incidence of 85% for clinically infected animals). The total microbial infection represented by 120 (60%) single bacterial isolates, 40 (20%) mixed bacterial isolates, 25 (12.5% fungi isolates and 15 (7.5%) mixed infection (bacterial and fungi). The most common aerobic microorganisms isolates were E.coli 19 (11.9%) followed by Croynebacterium pyogenes 15 (9.4%). The most common mixed bacterial isolates were E.coli + Croynebacterium pyogenes + Proteus spp with incidence of 22% and Staphylococcus aureus + Croynebacterium bovis with incidence of 20%. 25 fungi isolates (12.5%) from total microbial isolates were found. The most important fungi isolates were Aspergillus spp (24%) and Candida (24%). Fifteen cases out of 120 repeat breeder cases (12.5%) proved to have mixed infection (bacterial and fungal). The most common mixed infection caused by E.coli + Aspergillus spp (33.3%). After Sensitivity test, the most active antibiotics were Enerofluxacin, Oxytetracycline, Gentamycin and Nalidixic acid. Most bacterial isolates were resistant to Neomycin, Erythromycin and Ampicillin. Sensitivity test revealed that most bacterial isolates, in this study, were highly sensitive to Enerofluxacin, Oxytetracycline, Gentamycin and Nalidixic acid and resistant to Neomycin, Erythromycin and Ampicillin.

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