BEHAVIOUR OF CLOSTRIDIAL SPORES DURING A MODEL FISH PRESERVATION AND AT ABUSE TEMPERATURE

Behaviour of some Clostridial spores during a model fish preservation and at abuse temperature was investigated. The effects of various chemical
preservative solutions with other methods of fish preservation on the outgrowth of C.perfringens DSM 765, C.perfringens DSM 798 and C.bifermentans spores were studied. The preservative solutions (PS) were (i) nitrite curing salt (15%) with sodium ascorbate (0.5%), (ii) nitrite curing salt (25%) with sodium ascorbate (0.5%), (iii) sodium chloride (15%); potassium sorbate (0.5%): sodium benzoate (0.25%); ascorbic acid (0.5%) and acetic acid (1.5%). The other preservative methods used were partial dehydration (PD) and vacuum packaging. The results indicated that solution (iii) was the most efficient and rapid preservative in preventing spore outgrowth. The responses of the examined spores to all of the investigated preservative solutions and subsequent methods were similar at end of the fourth week of storage. The residues of the sodium nitrite, sodium nitrate, benzoate, sorbate, sodium chloride as well as pH and water activity were determined