EFFECT OF CRYSTAL VIOLET ON THE BIOLOGICAL CHARACTER OF C.ovis STRAINS

Document Type : Research article

Authors

Abstract

Eight strains of C.ovis from 4 animal species were treated with different dilutions of crystal violet to detect its effect on their biological characters. Treatment with the subinhibitory dilution (1/250.000) caused a noticeable variations in their cultural character, biochemical reactions, and pathogenicity to g.pigs. Vaccinations of 4 groups of 9.pig with each of the 4 C.v. attenuated strains gave 91.33% survival. Also challenge by intraocular instillation of virulent SM sheep strain showed that the g-pigs vaccinated with the C.V. buffalo, sheep and goat strains developed observable protection, which prevented completely or partially the development of lesions in the submaxillary lymph nodes and minimize the mortality rate in comparison to the positive controls. Elsewhere, g.pigs vaccinated with C.v. cattle strain induced partial immunity by prolonging the time of survival.