PRELIMINARY STUDIES ON THE APPLICATION OF SOME RECENT TECHNIQUES IN THE DETECTION OF RINDERPEST VIRAL ANTIGENS AND ANTIBODIES

MADBOLY, H.M.; EL-SANOUSI, A.; OSMAN, K.M.; OSMAN, O.A.; SABER, M.S.; REDA, I.M.

doi:10.21608/avmj.1987.189531

PRELIMINARY STUDIES ON THE APPLICATION OF SOME RECENT TECHNIQUES IN THE DETECTION OF RINDERPEST VIRAL ANTIGENS AND ANTIBODIES

Document Type : Research article

Authors

10.21608/avmj.1987.189531

Abstract

In this work, some of the recently applied serological techniques, have been developed and evaluated for he detection of rinderpest viral antigens and antibodies. Different methods have been adopted for the preparation of agar gel precipitating antigen. It has been proved that the NP-40 cell-extracted viral antigen is potent more reliable for detection of rinderpest viral antigens in tissue culture. SPA (staph. aureus Protein-A)-agglutination test wa used as a slide and microplate assay and found to be easier, rapid and more sensitive than the Agar Gel Precipitation test (AGPT). Parallel to the above two techniques, the Dot-Immunobinding Assay (Dot ELISA) has proved to be the most sensitive and versatile technique in detecting rinderpest viral antigen in tissue culture. In combination with the above mentioned methods, the titers of antibodies found in rabbit-anti- Rinderpest serum were estimated using the serum neutralization test and the cell-ELISA. It could be concluded that the cell-ELISA is a very sensitive reliable test which could be easily performed for the determination of antibody titers. A notable progress has been achieved for the detection of rinderpest viral antigens in organs and lymph nodes of naturally infected animals using the SPA-agglutination and Dot-ELISA test besides the AGPT.