EFFECT OF STARVATION, DARKNESS AND CONTINUOUS LIGHT ON TOTAL PROTEINS AND PROTEIN FRACTIONS IN BLOOD OF SERUM OF THE LIZARD, CHALCIDES OCELLATUS

Document Type : Research article

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Abstract

The effect of starvation, darkness and exposure to continuous light for 60 days on total proteins and protein fractions in blood serum of the lizard, Chalcides ocellatus was investigated. Total proteins and protein fractions were determined applying the Biruet method, and horizontal zone electrophoresis on agarose slide, respectively. Quantitative changes in total proteins and protein fractions namely: albumins, alpha globulin, beta globulin and gamma globulin in the serum of the lizard before and after exposure to starvation, darkness and continuous light were assess ed. The results revealed that no qualitative changes were detected in these protein fractions, however marked quantitative changes in the serum of the lizard were recorded after the exposure to such environmental conditions.