A TRIAL TO DETERMINE AGE OF WOUND AFTER PLASTIC MARK APPLICATION IN COWS AND BUFFALOE'S EARS

The sequence of the healing process was studied in 24 cows and buffa loes after application of both insurance and vaccination plastic marks. It had been observed that, during the first three days (24-95 hrs), the most important histopathological changes were haemorrhage, oed ema, necrosis and leucocytic infiltration inostly of neutrophil cell type. One week after application of the mark, healing was started in dermis with formation of vascular connective tissue which was mostly infiltra ted with inflamatory cells). Two weeks after application of the mark, regeneration of the epithelium was started. Three weeks after applica tion of the mark, healing of severed auricular cartilage was observed. Complete healing of the dermis and epidermis was observed in cows, four weeks after application of the marks. However in buffaloe epithel - ization was incomplete at this stage. Six weeks after application of the mark, healing with complete epithelization was observed in both buffaloes and cows. Eight weeks up to six months, connective scar become avascular and contracted, the area was depressed under the surface and was devoid of adnexal structure (hair follicles, sweet and sebacious glands).
it was concluded that, study the sequence of changes of healing process occuring in the ear of buffaloe and cows after application of the vaccination or insurance mark may be of great help in determination of the time elapsed after its application.