IN VITRO IDENTIFICATION OF NAEGLERIA AND ACANTHAMOEBA ISOLATED FROM WATER AND SEAGE

Document Type : Research article

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Abstract

Potentially pathogenic free-living amoebae of the genera Naegleria and Acanthamoeba were isolated from the Egyptian aquatic environment (water and sewage). The isolated species of both genera were identified on the basis of morphological characteristics, type and form of growth in axenic SCGYEM medium and on monoxenic NM medium as well as the maximum temperature tolerance. The study revealed the identification of four species of Naegleria and six species of Acanthamoeba. SCGYEM medium was found to be more favourable for testing the pathogenicity of Naegleria only, while NM medium was favourable for testing both Naegleria and Acanthamoeba species.

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