COMPARISON OF CONVENTIONAL STAINING TECHNIQUES AND FITC-LABELED MONOCLONAL ANTIBODY-BASED METHOD FOR DETECTION OF CRYPTOSPORIDIUM OOCYSTS IN NEONATAL CALVES

SALEM, G.H.,; NESSIEM, M.G.,; YACOUP, R.S.,

doi:10.21608/avmj.1994.185555

COMPARISON OF CONVENTIONAL STAINING TECHNIQUES AND FITC-LABELED MONOCLONAL ANTIBODY-BASED METHOD FOR DETECTION OF CRYPTOSPORIDIUM OOCYSTS IN NEONATAL CALVES

Document Type : Research article

Authors

10.21608/avmj.1994.185555

Abstract

This study was assessed to discover the ability of four staining techniques, initially, developed for the rapid identification of Cryptosp < /em>oridium sp < /strong>ecies oocysts in faecal samples of 55 neonatal calves less than 2 weeks old. All the stains employed in this study (modified Ziehl-Neelsen, Safranin-methylene blue, Giemsa and FITC-labeled monoclonal antibody as a commercial kit) detected the oocysts with incidence discrepancy. Concentration flotation of the samples raised diagnostic value of all techniques used. FITC labeled monoclonal antibody was the best in identifing the oocysts from concentrated faecal samples (72.7%) as well as from the unconcentrated one (63.6%). The recovery of oocysts by other methods was variable includes modified Zeihl-Neelsen (45.5%), safranin-methylene blue (36.4%) and Giemsa stain (18.2%). Our findings suggested that FITC-labeled monoclonal antibody as a probex to detect the oocyst specific epitope even without concentration flotation was highly sensitive and specific than the other traditional techniques. Combination between fluorescent antibody and modified Ziehl-Neelsen stains, where possible, are the currently recommended procedures for the detection of oocysts.