PREVALENCE OF PSEUDOMONADS IN FARM BULK-MILK

Document Type : Research article

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Abstract

One hundred and fifty random raw milk samples were collected weekly from farm bulk-milk tank at different private and governomental dariy farms at El Mansoura city, Egypt. The samples were bacteriologically examined for enumeration and isolation of pseudomonas organisms as well as detection of proteolytic and lipolytic activity of the isolates. Pseudomonads were detected in 89.3% milk samples with a mean count of 4.9x104 cfu/ml on Pseudomonas selective medium (PSM), while the incidence was 98.7% with
a mean count of 2.2x105 cfu/ml on Pseudomonas Aeromonas medium (PAM). Accepted correlation was computed between the two media (r=0.62). Eight species of pseudomonas could be isolated. PAM gave a higher frequency of false positive competitors than PSM. Out of 215 pseudomonas isolates, 177 and 163 were produced proteolytic and lipolytic activity respectively.

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