AGENTS INCREMINATED IN MICROBIA REDUCED HATCHABILITY OF DUCK EMBRYOS II. ANTIMICROBIAL IN-VITRO SENSITIVITY TESTING OF Salmonella emek AND E. coli ISOLATES USING MINIMUM INHIBITORY CONCENTRATION (MIC)

Document Type : Research article

Authors

1 Animal Health Research Laboratory, Assiut

2 Dept. Poultry Diseases, Fac. Vet. Med., Assiut Univ.

Abstract

Thirteen isolates of Salmonella emek, and Eleven isolates of E. coli, were tested for their susceptibility to ten antimicrobial agents using MIC technique. Concerning Salmonella emek, 92.3% of isolates were resistant to the action of sulfademidine with MIC value of > 50 ug/ml. All isolates were completely susceptible to the action of flumequine, enrofloxacine, amoxacilline, oxytetracycline, chlortetracycline, erythro mycin, neomycin, nalidixic acid and colistin sulphate with MIC values of 0.2, 0.2, 0.2, 0.4, 0.4, 0.78, 0.78, 1.56 and 1.56 ug/ml respectively. Four resistance patterns were noticed regarding E. coli isolates sensitivity testing. Colistin sulphate (18.2%) with MIC value of 6.25 ug/ml, nalidixic acid (54.5%) with MIC value ranged from 3.13 - 6.25 ug/ml, followed by 100% resistance of amoxacillin and sulfademidine with MIC values of 6.25 and 50 ug/ml. All isolates were completely susceptible to the action of enrofloxacine (MIC > 0.2 ug/ml), chlortetracycline and oxytetracycline (MIC > 0.4 ug/ml), erythromycin (MIC > 0.78 ug/ml), neomycin (MIC range of 0.4~0.78 ug/ml) and colistin sulphate (MIC > 1.56 ug/ml).

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