RAPID DETECTION OF CONGO-CRIMEAN HAEMORRHAGIC FEVER ANTIBODIES

Document Type : Research article

Author

Dept. of Virology, Animal Health Research Institute, Dokki, Giza

Abstract

Serum sample from 3985 apparently healthy sheep and goats were tested for CCHF by indirect immunofluorescence (IF) and Dot-enzyme linked immunosorbent assay (Dot-ELISA). Infected inactivated Vero cell suspension was used as antigen in the study. CCHF viral antibody prevalence was found among sheep (2%), while not among goats. The
and 13% in age >1 year and < percentages of reactive sera were 87% lyear subsequently. Neither IgM antibody in sera nor CCHF viral antigen
in tick homogenates could be demonstrated. There was a correlation between Dot-ELISA and IF not only with the respect to the total numbers of positives, and negatives, but also, for each individual serum. Moreover, Dot-ELISA was more sensitive than IF, but needed more time for application. So IF technique was faster especially when rapid result delivery had been highly recommended.

Keywords