In this study, inactivated Bovine Rota virus (BRV) vaccine was evaluated in five susceptible vaccine. Each animal was vaccinated intramuscularly (IM) with two doses of 2 ml of the vaccine (3 weeks apart). No clinical signs of illness as post-vaccination (PV). No shedding of the virus through secreta and excreta. Specific BRV neutralizing antibodies developed early at the 10th day PV. The antibodies were monitored till the 7th month PV. The developed antibodies were quantitavely measured by both Serum Neutralization Test (SNT), and Enzyme Linked ImmunoSorbent Assay (ELISA).