VALIDITY OF GNRH AND MELATONIN INTERACTION ON SOME REPRODUCTIVE PERFORMANCE OF BARKI RAMS

12 Sexually, mature Barki rams were used. They were divided into four comparable groups (3 rams/each), Group (1) was injected S/C twice daily by 1.5 mg melatonin, then injections were repeated at 3rd and 7th day. Group (2) was injected by 125 ug GnRH I/M at the same regimen of group 1. Group (3) was injected by melatonin at the 1st and 3rd day than followed by GnRH injection as in the first two groups. Group (4) was injected by GnRH followed by melatonin as the third group. Blood and semen samples were collected before and all over the period of treatment and persist till 9 weeks as post treated period. Semen samples were evaluated for semen volume, sperm concentration, percentages of live spermatozoa individual motility and total sperm abnormalities. Scrotal circumference and reaction time were measured. Plasma FSH, LH and testosterone were also determined. The obtained results revealed that melatonin injection (Group 1) was significantly increased the percentages of live spermatozoa, sperm concentration, scrotal circumference, reaction time and total sperm abnormalities. Also the levels of FSH, LH and testosterone were increased. While GnRH injection (group 2) decreased significantly the semen volume, reaction time and sperm concentration but with a significant increase in percentage of line spermatozoa, total sperm abnormalities sperm concentration and testosterone secretion. Injection of melatonin followed by GnRH (group 3) showed a significant decrease in percentage of individual motility and testosterone. While injection of GnRH followed by melatonin (group 4) revealed a significant increase in percentage of live spermatozoa, FSH and testosterone with decrease in LH level. The reaction time increased then return back to the normal. It was concluded that the use of melatonin after GnRH treatment are validated for reproductive improvement of Barki rams.
12 Sexually, mature Barki rams were used. They were divided into four comparable groups (3 rams/each), Group (1) was injected S/C twice daily by 1.5 mg melatonin, then injections were repeated at 3rd and 7th day. Group (2) was injected by 125 ug GnRH I/M at the same regimen of group 1. Group (3) was injected by melatonin at the 1st and 3rd day than followed by GnRH injection as in the first two groups. Group (4) was injected by GnRH followed by melatonin as the third group. Blood and semen samples were collected before and all over the period of treatment and persist till 9 weeks as post treated period. Semen samples were evaluated for semen volume, sperm concentration, percentages of live spermatozoa individual motility and total sperm abnormalities. Scrotal circumference and reaction time were measured. Plasma FSH, LH and testosterone were also determined. The obtained results revealed that melatonin injection (Group 1) was significantly increased the percentages of live spermatozoa, sperm concentration, scrotal circumference, reaction time and total sperm abnormalities. Also the levels of FSH, LH and testosterone were increased. While GnRH injection (group 2) decreased significantly the semen volume, reaction time and sperm concentration but with a significant increase in percentage of line spermatozoa, total sperm abnormalities sperm concentration and testosterone secretion. Injection of melatonin followed by GnRH (group 3) showed a significant decrease in percentage of individual motility and testosterone. While injection of GnRH followed by melatonin (group 4) revealed a significant increase in percentage of live spermatozoa, FSH and testosterone with decrease in LH level. The reaction time increased then return back to the normal. It was concluded that the use of melatonin after GnRH treatment are validated for reproductive improvement of Barki rams.