COMPARATIVE STUDIES BETWEEN INDIRECT HEMAGGLUTINATION (IHA) TEST AND ENZYME LINKED IMMUNOSORBENT ASSAYS (ELISA) TEST FOR DETECTION OF SERUM ANTIBODY LEVELS AGAINST INFECTIOUS BURSAL DISEASE (IBD) VIRUS IN COMMERCIAL BROILERS

DAHSHAN, AL-H.M.; HUSSIEN, A.S.

doi:10.21608/avmj.2011.176945

COMPARATIVE STUDIES BETWEEN INDIRECT HEMAGGLUTINATION (IHA) TEST AND ENZYME LINKED IMMUNOSORBENT ASSAYS (ELISA) TEST FOR DETECTION OF SERUM ANTIBODY LEVELS AGAINST INFECTIOUS BURSAL DISEASE (IBD) VIRUS IN COMMERCIAL BROILERS

Document Type : Research article

Authors

AL-H.M. DAHSHAN ¹
A.S. HUSSIEN ²

¹ Dept. of Poultry Diseases, Faculty of Veterinary Medicine, Beni-Suef University

² Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University

10.21608/avmj.2011.176945

Abstract

This study was done to compare between IHA test and ELISA test as a serological test for detection of serum antibody levels against Infectious Bursal Disease Virus (IBDV) in Commercial Broilers, 300 Serum samples were collected from different commercial broiler farms and slaughter Shops from in and around Beni-suef Governorate. These samples were divided into three groups according to the age of the birds i.e. 0-3 weeks (100 samples), 3-6 weeks (100samples) and 6-8 weeks (100 samples).ELISA and Indirect Hemagglutination (IHA) test were used to titrate the serum antibodies against infectious bursal disease virus. Hyper immune serum was raised in laboratory rabbits against IBDV vaccine (D-78). It was used to identify and confirm the IBD virus from bursal tissues isolated from the IBD affected flocks using the qualitative tests i.e. agar gel precipitation suspension was prepared and used to determine the antibody titer. Results indicated that out of 300 test serum samples 195 (65%) were positive. The maximum positive samples belonged to 6-8 weeks of age (89%) followed by 3-6 weeks (73%) and 0-3 weeks (33%), respectively while the result of ELISA test of high and low titers were 18% and 82 % at 0-3 week of age then at age 3-6 weeks the high titers were 35% and low titers were 65% and finally high ELISA titers 69% and low titers 31%at age 6-8 weeks.

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