PATHOLOGICAL STUDIES ON THE EFFECT OF YEAST ON MYCOTOXICOSIS IN RATS

Authors

1 Pathology Department, Animal Health Institute

2 Pathology Department, Faculty of Veterinary Medicine, Assiut University

3 Botany Department, Faculty of Science, Assiut University

Abstract

The present study aimed to investigate the protective effect of yeast against mycotoxicosis induced by Aspergillus parasiticus and Fusarium tricinctum as common fungal contaminants on albino rats. 60 albino rats were randomly divided into three experimental groups: (A, B and C), each contain 20 animals. Group A: rats were kept as a control group was feed on uncontaminated feed and drinking water without any treatments. Group B: animals were feed contaminated diet with aflatoxins in level of 0.5 mg/kg ration and diacetoxyscirpenol in level of 10mg/kg ration. Group C: animals were feed contaminated diet as in group B. mixed with Saccharomyces cerevisiae (2g/kg of feed) during the whole time of the experiment. At the end of 1st, 2nd, 3rd, and 4th month, respectively, five animals from each group were weighted and dissected. Tissue samples were obtained from liver, kidneys and intestine for histopathological examination by light and electron microscope. The rats showed reduction of body weight and weight gain in group B. Addition of yeast to contaminated diet in the group C improved this reduction. Histopathological and ultrastructural studies revealed pathological changes in liver and kidney in group B. administration of yeast improve the intensity and the prevalence of the lesions and enhances the immune response of the body against mycotoxicosis (Lymphocytes and plasma cells).
 

Keywords


Pathological, Yeast, Mycotoxicosis, Rats.

 

 

Abousadi, A.M.; Rowghani, E. and Honarmand, M.E. (2007):The efficacy of various additives to reduce the toxicity of aflatoxin B1 in broiler chicks. Iranian Journal of Veterinary Research 8: 144-150.
Baptista, S.; Abdalla, L.; Aguiar, L.; Baptista, D.; Micheluchi, S.; Zampronio, C.; Pires, S.; Glöria, M.; Calori-Domingues, A.; Walder, M.; Vizioli, R. and Horii, J. (2008):Utilization of diets amended with yeast and amino acids for t he control of aflatoxicosis. World Journal of Microbiology and Biotechnology 24: 2547–2554.
Basmacioglu, H.; Oguz, H.; Ergul, M.; Col, R. and Birdane, Y.O. (2005):Effect of dietary esterified glucomannan on performance, serum biochemistry and haematology in broilers exposed to aflatoxin. Czech Journal of Animal Science 50: 31–39.
Betina, V. (1989):Biological effects of mycotoxins. In: V. Betina (Ed), Mycotoxins: Chemical, Biological and Environmental Aspects. Elsevier, Amsterdam. PP: 42-58.
Brown, T.P.; Fletcher, O.J.; Osuna, O. and Wyatt, R.D. (1987):Microscopic and ultrastructural renal pathology of oosporein-induced toxicosis in broiler chicks. Avian Diseases 31: 868-877.
Buts, J.P.; Bernasconi, P.; Vaerman, J.P. and Dive, C. (1990):Stimulation of secretory IgA and secretory component of immunoglobulins in small intestine of rats treated with Saccharomyces boulardii. Dig. Dis. Sci. 35: 251-256.
Casado, J.M.; Theumer, M.; Masih, D.T.; Chulze, S. and Rubinstein, H.R. (2001):Experimental subchronic mycotoxicoses in mice: individual and combined effects of dietary exposure to fumonisins and aflatoxin B1. Food and Chemical Toxicology 39: 579-586.
Çelik, I.; Oğuz, H.; Demet, Ö.; Dönmez, H.H.; Boydak, M.; Sur, E. (2000):Efficacy of polyvinylpolypyrrolidone in reducing the immunotoxicity of aflatoxin in growing broilers.  Br. Poult. Sci. 41: 430–439.
Chen, F.; Ma, Y.; Xue, C.; Ma, J.; Xie, Q.; Wang, G.; Bi, Y. and Cao, Y. (2008):The combination of deoxynivalenol and zearalenone at permitted feed concentrations causes serious physiological effects in young pigs. J. Vet. Sci. 9: 39-44.
Colakoglu, F. and Donmez, H.H. (2012):Effects of Aflatoxin on Liver and Protective Effectiveness of Esterified Glucomannan in Merino Rams. The Scientific World Journal Volume 2012, Article ID 462925, 5 pages.
Darwish, H.R.; Omara, E.A.; Abdel-Aziz, K.B.; Farag, I.M.; Nada, S.A. and Tawfek, N.S. (2011): Saccharomyces cerevisiae modulates Aflatoxin-induced toxicity in male Albino mice. Report and Opinion 3: 32-43.
Del Bianchi, M.; Oliveira, C.A.F.; Albuquerque, R.; Guerra, J.L. and Correa, B. (2005):Effects of prolonged oral administration of aflatoxin B1 and fumonisin B1 in broiler chickens. Poult.  Sci. 84: 1835–1840.
Dhanasekaran, D.; Panneerselvam, A. and Thajuddin, N. (2009):Evaluation of aflatoxicosis in hens fed with commercial poultry feed. Turk. J. Vet. Anim. Sci. 33: 358-391.
Dorner, J.W. (2008):Management and prevention of mycotoxins in peanuts.  Food   Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess. 25: 203–208.

Fairchild, A.S.; Grimes, J.L.; Porter, J.K.; Croom Jr., W.J.; Daniel, L.R. and Hagler, Jr., W.M. (2005): Effects of diacetoxyscirpenol and fusaric acid on poults: individual and combined effects of dietary diacetoxyscirpenol and fusaric acid on turkey poult performance. Int. J. Poult. Sci. 4: 350–355.

Gimeno, A. (1979):Thin layer chromatographic determination of aflatoxins, ochratoxins, sterigmatocystin, zearalenone, citrinin, T-2 toxin, diacetoxyscirpenol, penicillic acid, patulin and penitrem A. J. Assoc. Offic. Anal. Chem. 62: 579-585.
Girish, C.K. and Smith, T.K. (2008):Effects of feeding blends of grains naturally contaminated with Fusarium mycotoxins on small intestinal morphology of turkeys. Poultry Science 87: 1075–1082.
Gopinath, C.; Prentice, D.E. and Lewis, D.J. (1987):The liver. In Atlas of Experimental Toxicological Pathology (G.A. Grasham, ed.), Vol. 13, pp. 43–60. MTP Press Limited, Lancaster, England.
Gounalan, S.; Balachandran, C. and Manohar, B.M. (2005):Immunopathological effects and induction of apoptosis in spleen and thymus in layer chicken in aflatoxicosis. International conference on advance Veterinary practice in medicine and surgery augmenting health and production, Chennai, 21-25 June, 2006.
Hashem, M.A. and Mohamed, M.H. (2009):Haemato-biochemical and pathological studies on aflatoxicosis and treatment of broiler chicks in Egypt. Veterinaria Italiana 45: 323- 337.
Hoerr, F.J. (1998):Pathogenesis of enteric diseases. Poult. Sci. 77: 1150–1155.
Hoerr, F.J.; Carlton, W.W.; Yagen, B. and Joffe, A.Z. (1982):Mycotoxicosis caused by either T-2 toxin or diacetoxyscirpenol in the diet of broiler chickens. Fund. Appl. Toxicol. 2: 121–124.
Hussain, S.; Khan, M.Z.; Khan, A.; Javed, I. and Asi, M.R. (2009):Toxico-pathological effects in rats induced by concurrent exposure to aflatoxin and cypermethrin. Toxicon 53: 33-41.
Hussain, Z.; Khan, M.Z. and ul-Hassan, Z. (2008):Production of aflatoxins from Aspergillus flavus and acute aflatoxicosis in young broiler chicks. Pak. J. Agri. Sci. 45: 95-102.
Ihara, T.; Sugamata, M.; Sekijima, M.; Okumura, H.; Yoshino, N. and Ueno, Y. (1997):Apoptotic cellular damage in mice after T-2 toxin-induced acute toxicosis. Natural Toxins 5: 141–5.
Jones, G. and Butler, W.H. (1975):Morphology and spontaneous neoplasia. In Mouse Hepatic Neoplasia (W.M. Butler and P.M. Newberne, eds.), pp 21–59. Elsevier, Oxford, UK.
Jouany, J.P. (2007):Methods for preventing, decontaminating and minimizing the toxicity of mycotoxins in feeds. Animal Feed Science and Technology 137: 342–362.
Kana, J.R.; Teguia, A. and Tchoumboue, J. (2010): Effect of dietary plant charcoal from Canarium schweinfurthii Engl. and maize cob on aflatoxin B1 toxicosis in broiler chickens. Adv.  Anim. Biosci. 1, 462–463.
Kumar, P.A.; Satyanarayana, M.L.; Vijayasarathi, S.K.; Gowda, R.N.S. and Rao, S. (2003):Pathology of lymphoid organs in aflatoxicosis and ochratoxicosis and immunomodulatory effect of vitamin E and selenium in broiler chicken. Indian Journal of Veterinary Pathology 27: 102-106.
Kumar, R. and Balachandran, C. (2009):Histopathological changes in broiler chickens fed aflatoxin and cyclopiazonic acid. Vet. Arhiv. 79: 31–40.
Madrigal-Santillán, E.; Madrigal-Bujaidar, E.; Márquez-Márquez, R. and Reyes, A. (2006): Antigenotoxic effect of Saccharomyces cerevisiae on the damage produced in mice fed with aflatoxin B1contaminated corn. Food and Chemical Toxicology 44: 2058–2063.
Métailler, R. and Huelvan, C. (1993): Uitilisation des levures dans l'alimentation du juvénile de bar (Dicentrarchus labrax). In: Kaushik, S. and Luquet, P. (Eds.): Fish Nutrition in Practice Les Colloques, Institut National de la Recherche Agronomique, Paris, 61: 945– 948.
Mohamed, N.A. (1996):Experimental studies on the combined effect of some amino acids, minerals and vitamins on the pathological changes of aflatoxin B1 in albino rats. M.V.Sc. Degree in vet. Pathology and clinical pathology, Assiut University.
Moslehi-Jenabian, S.; Pedersen, L.L. and Jespersen, L. (2010):Beneficial effects of probiotic and food borne yeasts on human health. Nutrients 2: 449-473.
Newberne, P.M.; Carlton, W.W. and Wogan, G.N. (1967):Hepatomas in rats and hepatorenal injury in ducklings fed peanut meal or Aspergillus flavus extract. Pathol. Vet. 1: 105-132.
Newman, K.E. (1994):Mannanoligosaccharide, natural polymers with significant impact on the gastrointestinal microflora and immune system. In: Biotechnology in the feed industry. T.P. Lyons and K.A. Jaques (eds.). Kentucky: Alltech Inc. pp: 167-174.
Oguz, H. (1997):The preventive efficacy of polyvinylpolypyrrolidone (PVPP) alone and its combination with the other adsorbents into broiler feeds against aflatoxicosis. Ph. D. Thesis. Institute of Health Sciences, University of Selçuk, Konya.
Oliva-Teles, A. and Gonçalves, P. (2001): Partial replacement of fish meal by brewer’s yeast (Saccharomyces cerevisiae): in diets for sea bass (Dicentrarchus labrax): juveniles. Aquaculture 202: 269–278.
Onifade, A.; Obiyan, R.; Onipede, E.; Adejumo, D.; Abu, O. and Babatunde, G. (1999):Assessment of the effects of supplementing rabbit diets with a culture of Saccharomyces cerevisiae using growth performance, blood composition and clinical enzyme activities. Anim. Feed Sci. Technol. 77: 25–32.
Ozen, H.; Karaman, M. and Cigremis, Y. et al., (2009):Effectiveness of melatonin on aflatoxicosis in chicks. Research in Veterinary Sciences 86: 485–489. 2009.
Parlat, S.S.; Ozcan, M.H. and Oguz, H. (2001):Biological Suppression of aflatoxicosis in Japanese quail (Coturnix coturnix japonica) by dietary addition of yeast (Saccharomyces cerevisiae). Research in Veterinary Science 71: 207-211.
Pestka, J.J. (2003):Deoxynivalenol-induced IgA production and IgA nephropathy-aberrant mucosal immune response with systemic repercussions. Toxicol. Lett. 140-141: 287–295.
Placinta, C.M.; D'Mello, J.P.F. and Macdonald, A.M.C. (1999):A review of worldwide contamination of cereal grains and animal feed with Fusarium mycotoxins. Animal Feed Science and Technology 78: 21-37.
Quezada, T.; Cuellar, H.; Jaramillo-Juarez, F.; Valdivia, A.G. and Reyes, J.L. (2000):Effects of aflatoxin B(1) on the liver and kidney of broiler chickens during development. Comp. Biochem. Physiol.C Toxicol. Pharmacol. 125: 265–272.
Quist, C.F.; Bounous, D.I.; Kilburn, T.V.; Nettles, V.F. and Wyatt, R.D. (2002):The effect of dietary aflatoxin on wild turkey poultry. J. Wildl. Dis. 36: 436-444.
Romer, R.T. (1975):Qualitaitve/quantitiatve analysis for detection and estimation of aflatoxin (Romer mini column method). J. Assoc. Off. Anal. Chem. 58: 500-506.
Saddiq, A.A. and Kalifa, S.A. (2011):Impact of fungal content of some Arabic nuts to induce kidney toxicity and agonistic action of natural resources. African Journal of Microbiology Research 5: 1046-1056.
Sakr, S.A.; Lamfon, H.A. and El-Abd, S.F. (2006):Ameliorative effect of Lupinus seeds on histopathological and biochemical changes induced by Aflatoxin-B1 in rat liver. Journal of Applied Science Research 2: 290-295.
Santin, E.; Paulilo, A.C.; Maiorka, A.; Nakaghi, L.S.O.; Macan, M. and de Silva, A.V.F., et al. (2003): Evaluation of the efficiency of Saccharomyces cerevisiae cell wall to ameliorate the toxic effects of aflatoxin in broilers. International Journal of Poultry Science 2: 241–344.
Savage, T.F.; Cotter, P.F. and Zakrzewska, E.I. (1996):The effect of feeding mannan oligosaccharide on immunoglobulins, plasma IgG and bile IgA of Wordstad MW male turkeys.  Poultry Science 75: 53-64.

Shotwell, O.L.; Hesseltine, C.W.; Stubblefield, R.D. and Sorenson, G.W. (1966): Production of aflatoxin on rice. Appl. Microbiol. 14: 425-428.

Sklan, D.; Klipper, E. and Friedman, A. (2001):The effect of chronic feeding of Diacetoxyscirpenol, T-2 toxin, and Aflatoxin on performance, health, and antibody production in chicks. J. Appl. Poultry Res. 10: 79–85.
Sklan, D.; Shelly, M.; Makovsky, B.; Geyra, A.; Klipper, K. and Friedman, A. (2003):The effect of chronic feeding diacetoxyscirpenol and T-2 toxin on performance, health, small intestinal physiology and antibody production in turkey poults. Br. Poult. Sci. 44: 46–52.
Smith, J. and Ross, K. (1991):Detoxigenic aspergilli. In: Smith, J.E., Henderson, R.S. (Eds.), Mycotoxins and Animal Foods. CRC Press, Boca Raton, pp. 101–118.
Speijers, G.J.A. and Speijers, M.H.M. (2004):Combined toxic effects of mycotoxins. Toxicology Letters 153: 91–98.
Valdivia, A.G.; Martinez, A.; Damian, F.J.; Quezada, T.; Ortiz, R.; Martinez, C.; Llamas, J.; Rodriguez, M.L.; Yamamoto, L.; Ja-ramillo, F.; Loarca-Pina, M.G. and Reyes, J.L. (2001):Efficacy of N-acetylcysteine to reduce the effects of aflatoxin B1 intoxication in broiler chickens.  Poult. Sci. 80: 727–734.
Van Egmond, H.P. and Jonker, M.A. (2004):Worldwide regulations on aflatoxins-the situation in 2002. J. Toxicol. Toxin Rev. 23: 273–293.
Wang, J-S.; Busby, W.F.Jr. and Wogan, G.N. (1990):Comparative tissue distribution and excretion of orally administered [3H] diacetoxyscirpenol (anguidine) in rats and mice. Toxicol. Appl. Pharmacol. 103:430-40.
Wannop, C.C. (1961):The histopathology of turkey "X" disease in Great Britain. Avian   Dis. 5: 371-381.
Yener, Z.; Celik, I.; Ilhan, F. and Bal, R. (2009):Effects of Urtica dioica L. seed on lipid peroxidation, antioxidants and liver pathology in aflatoxin-induced tissue injury in rats. Food and Chemical Toxicology 47: 418–424.
Yildiz, A.O.; Parlat, S.S. and Yildirim, I. (2004):Effect of dietary addition of live yeast (Saccharomyces cerevisiae) on some performance parameters of adult Japanese quail (Coturnix coturnix japonica) induced by aflatoxicosis. Revue Méd. Vét. 155: 38-41.
Yousef, J.M. (2009):The Protective Effects of Selected Mixed Herbal Extracts on Weight, Serum and Liver Tissue in Rats Before and after Exposure to Aflatoxin B1. World J. Med. Sci. 4 (1): 01-08.
Yunus, A.W.; Razzazi-Fazeli, E. and Bohm, J. (2011):Aflatoxin B1 in affecting broiler’s performance, immunity, and gastrointestinal tract: a review of history and contemporary Issues. Toxins 3: 566-590.
Zanello, G.; Meurens, F.; Berri, M. and Salmon, H. (2009):Saccharomyces boulardii effects on gastrointestinal diseases. Curr. Issues Mol. Biol. 11: 47–58.