HISTOLOGICAL, HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL STUDIES ON THE PARAVENTRICULAR AND SUPRAOPTIC NUCLEI OF THE HYPOTHALAMUS IN THE ADULT NEW ZEALAND RABBITS

Document Type : Research article

Authors

Department of Cytology and Histology, Faculty of Veterinary Medicine, Beni-Suef University

Abstract

The present investigation aims to give a detailed histomorphological feature of the anterior group of hypothalamus of adult New Zealand rabbits in both sexes. Specimens were prepared to be examined microscopically by using light and transmission electron microscope. The obtained results revealed that, the anterior group of the hypothalamus composed of paraventricular (PVN) and supraoptic nuclei (SON). The paraventricular nucleus was located on either sides of the 3rd ventricle. It consisted of different-shaped neurons which appeared in the form of clusters and irregular groups intermingled with nerve fibers associated with neuroglia cells and permeated by blood capillaries. Ultrastructurally, the neurons appeared polyhedral in shape contained large eccentric euchromatic nucleus and electron lucent cytoplasm contained many mitochondria, rER, Golgi apparatus, numerous ribosomes and some secretory granules. On the other hand, the supraoptic nucleus was located dorsolateral to the optic chiasma and consisted of two portions; thin medial and thick lateral one. The neurons of the thin medial part appeared pyramidal in shape with vesicular oval eccentric nuclei and lightly stained cytoplasm, while that of the thick lateral one showed irregular groups or rows of oval and pyriform shaped cells contained vesicular rounded eccentric nuclei. Ultrastructurally, the neurons of the thick lateral part of SON appeared pyriform in shape with spherical eccentric euchromatic nucleus in electron lucent cytoplasm showed intensive network of rER, many mitochondria, Golgi apparatus, numerous free ribosomes and few secretory granules. The cytoplasmic granules of the PVN and SON neurons gave positive reaction with all nonspecific and specific stains used. Also, they gave positive immunoreactions when subjected to Anti-CGA and Anti-NSE antibodies. There were no microscopic structural differences correlated to neither sex nor age of studied animals.

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References

REFERENCES
 
Adams, C.W.M. and Sloper, J.C. (1955): Technique for demonstrating  neurosecretory material in human hypothalamus. Lancet, 26, 651-652.
Adams, C.W.M. and Sloper, J.C. (1956): The hypothalamic elaboration of Posterior pituitary principles in man, the rat and dog. Histochemical         evidence derived from a performic acid-alcian bluereaction for cystine. J. Endocr., 13, 221-222.
Ambach, G. and Palkovits, M. (1979): The blood supply of the hypothalamus in the rat. In anatomy of the hypothalamus. Vol. 1, ed. Morgane P.J. and Panksepp J., 263-377. New York: Marcell, Dekker.
Bancroft, G.D. and Stevens, A. (1996): Theory and practice of histological techniques. 4th Ed., Churchill-Livingstone, Edinburgh,   London, Melborne, New York.
Bancroft, J. and Gamble, A. (2008): Theory and practice of histological techniques. 6th Ed. Churchill-Livingstone, Edinburgh, London, Melbourne, New York.
Bargmann, W. (1949): Sober die neurosekretorische verknupfung von   hypothalamus und neurohypophyse. Z. zellforsch. 34, 610-634. cited by Sloper (1989).
Barry, I. (1975): Essai de classification, entechnique de Golgi, des diverses          categories de neurones de noyauparaventriculaire chez la souris. C.R. Seances Soc. BioL., 169: 978-980.
Bazhanova, E.D.; Grinevich, V.V. and Danilova, O.A. (1998): Neuroscience and Behavioral Physiology., 28(6): 26–29.
Bisset, G.W.; Clark, Barbara, J. and Haldar, Jaya (1970): Blood levels of oxytocin and vasopressin during suckling in the rabbit and the problem of their independent release. J. Physiol. 206, 711-722.
Bõzse, Z.S. and Houdebine, L.M. (2006): Application of rabbits in biomedical research: A Review World Rabbit Sci., 14, 1–14.
Cetin, Y. (1992): Chromogranin A immunoreactivity and Grimelius' argyrophilia. Anat. Embryol. 185(3): 207-215.
Cotea, C.; Oprean, O.Z.; Boişteanu, P.; Solcan, Carmen, and Cotea, I. (2007): The nuclei of the hypothalamus in cow. Cercetări Agronomiceîn Moldova 4(132), 71–79.
De la Portilla, F.; López-Alonso, M.; Borrero, J.J.; Diaz-Pvon, J.; Gollonet, J.L.; Palacios, C.; Vazquez-Monchul, J. and Sánchez-Gil, J.M. (2011): The rabbit as an animal model for proctology research: anatomical and histological description. J. Investig. surg., 24(3): 134-137.
Duan, L.; Yuan, H.; Su, C.; Liu, Y. and Rao, Z. (2004): Ultrastructure of junction areas between neurons and astrocytes in rat supraoptic nuclei, World J. Gastroenterol., 10(1): 117–121.
Felten, D.L. and Cashner, K.A. (1979): Cytoarchitecture of the supraoptic nucleus: A Golgi study. Neuroendocrinol., 29, 221-230.
Flament-Durand, D. and Dustin, E. (1972): Studies on the transport of secretory granules in the magnocellular hypothalamic neurons. Z.               Zellforsch. Mikrosk. Anat., 130(4), 440-454.
Fliers, E.; Swaab, D.F.; Pool, C.W. and Verwer R.W.H. (1985): The vasopressin and        oxytocin neurons in the human supraoptic and paraventricular nucleus: changes with aging and in senile dementia. Brain Res. 342: 45–53.
Freund-Mercier, M.J.; Stoeckel, M.E. and Klein, M.J. (1994): Oxytocin receptors on oxytocin neurones: histoautoradiographic detection in the lactating rat. J. Physiol., 480(1): 155-161.
Gomori, G. (1941): Observations with different stains on human islet of Langerhans. Amer. J. Pathol. 17: 395-406.
Graur, D.; Duret, L. and Gouy, M. (1996): Phylogenetic position of the order Lagomorpha (rabbits, hares and allies). Nature, 379, 333-335.
Gregory, W.A.; Tweedle, C.D. and Hatton, G.I. (1980): Ultrastructure of neurons in the paraventricular nucleus of normal dehydrated and rehydrated rats. Brain Research Bulletin, 5(3), 301–306.
Grimelius, L. (1968): A silver nitrate stain for α 2 cells in human pancreatic islets. Acta Soc. Med. upsalien., 73: 243-270.
Harris, H.F. (1900): A new method of ripening haematoxyline. (Cited by Bancroft and Gamble, 2008).
Hayat, M.A. (1986): Basic techniques for transmission electron microscopy, Academic Press, Jovnovich Publishers. Inc. New York, Harcourt Brace.
Huttner, W.B.; Gerdes, H.H. and Rosa, P. (1991): The granin (chromogranin/ secretogranin) family. Trends Biochem. Sci. 16, 27–30.
Hyyppä, M. (1969): Differentiation of the hypothalamic nuclei during ontogenetic development in the rat. Zeits Für Anat Und Entwick., 129(1): 41-52.
Hyyppä, M. (1969): Differentiation of the hypothalamic nuclei during ontogenetic development in the rat. Zeits Für Anat Und Entwick., 129(1): 41–52.
Kalimo, J. (1975): Ultrastructural studies on the hypothalamic neurosecretory neurons of the rat. Cell Tiss. Res., 163, 151–168.
Lassman, H.; Hagn, C.; Fusher-Colbrie, R. and Winkler, H. (1986):       Presence of CGA, B and C in bovine endocardium and nervous tissue; A     comparative immunohistochemical study; Histochem. J., 18, 380-386.
Leng, G.; Brown, C.H. and Russellin, J.A. (1999): Physiological pathways regulating the activity of magnocellular neurosecretory cells. Progress in Neurobiology, 57(6), 625–655.
Leontovich, T. (1970): The neurons of the magnocellular neurosecretory system of the dog's hypothalamus. A Golgi study. J. Him. orach., 11, 499-517.
Leveque, T.F. (1983): Changes in the neurosecretory cells of the rat hypothalamus following ingestion of sodium chloride. Anat. Rec., 117(4): 741-757.
Lundqvist, M.; Amberg, H.; Candell, J.; Malmgren, M.; Wilander, E.; Grimelius, L. and Oberg, K. (1990): Silver stains for identification of neuroendocrine cells: A study of the chemical background. Histochem. J.,22: 615-623.          
Machfn-Santamaria, C. (1978): Ultrastructure of the hypothalamic neurosecretory nuclei of the dormouse (Eliomysquercinus L.) in the awakening and. J. Anat., 127, 239–249.
Murray, G.I.; Duncan, M.E.; Melvin, W.T. and Fothergill, J.E. (1993):Immunohistochemistry of neuron specific enolase with subunit specific antipeptide monoclonal antibodies. J. Clin. Pathol., 46: 993-996.
Pearse, A.G.E. (1977): The diffuse neuroendocrine system and the APUD concept: related endocrine peptides in brain, intestine, pituitary, placenta and anuran cutaneous glands. Med. Biol., 55, 115-125.
Rao, S.S.; Sarojini, R. and Jayalakshmi, K. (1988): Histomorphology and histochemistry of the neurosecretory system in penaeid prawn, Metapenaeusaffinis. J. Adv. Zool., 9(2): 113-122.
Reynolds, E.S. (1963): The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol., 17: 208-212.
Richardson, K.C.; Jarett, L. and Finak, E.M. (1960): Embedding in epoxy resins for ultra-thin sectioning in electron microscopy. Stain Technol., 35: 313-323.
Saphier, D. and Feldman, S. (1985): Electrophysiologic evidence for neural connections between the paraventricular nucleus and neurons of the supraoptic nucleus in the rat. Exp. Neurol., 89, 289-294.
Scharrer, B. (1967): The neurosecretory neuron in neuroendocrine regulatory mechanisms. Amer. Zool., 7(1), 161–169.
Scharrer, E. and Scharrer, B. (1954): Hormones produced by neurosecretory      cells. Recent Progress, Hormone Res., 10, 183-240.
Schmechel, D.; Marangos, P.J. and Brigtman, M.W. (1978): Neuron specific enolase is a molecular marker for peripheral and central neuroendocrine cells. Nature, 276, 834-836.
Shek, J.W.; Wen, G.Y. and Wisniewski, H.M. (1986): Atlas of the rabbit brain and spinal cord. New York state office of mental retardation and developmental disabilities, Institute of basic research in developmental disabilities, Staten Island, N. Y., USA.
SHI, L.; FAN, Y. and XU, Z. (2012): Development of Oxytocin- and Vasopressin Network in the Supraoptic and Paraventricular Nuclei of Fetal Sheep. Physiol. Res. 61: 277-286.
Sloper, J.C. (1989): Hypothalamic neurosecretion in the dog and cat, with particular reference to the identification of neurosecretory material with posterior lobe hormone. J. Anat., 20(2): 301–316.
Sofroniew, M.V.; Weindl, A.; Schinko, I. and Wetzstein, R. (1979): The distribution of vasopressin oxytocin and neurophysin producing neurons in the guinea pig brain. Cell Tiss. Res., 196, 367-384.
Solcia, E.; Vassallo, G. and Capella, C. (1968): Selective staining of endocrine cells by basic dyes after acid hydrolysis. Stain Technol., 43, 257-263.
Stafford, L. and Lightman, B.Y. (1988): The neuroendocrine paraventricular hypothalamus: Receptors, signal transduction, mRNA and neurosecretion. J. Exp. Biol., 49, 31–49.
Swaab, D.F. and Fliers, E. (1985): A sexually dimorphic nucleus in the human brain. Science, 228, 1112–1114.
Taupenot, L.; Harper, K.L. and O’Connor, D.T. (2003): The chromogranin-secretogranin family. N. Engl. J. Med. 348, 1134–1149.
Wand, G.S.; Takiyyuddin, M.; O'Connor, D.T. and Levine, M.A. (1991): A proposed role for CGA as a glucocorticoid responsive autocrine inhibitor of proopiomelanocortin secretion. Endocrinol., 128, 1345-1351.
Watkins, W.B. (1975): Immunohistochemical demonstration of neurophysin        in the hypothalamo-neurohypophysial system. Int. Rev. Cytol. 41: in press.
Zaborszky, L.; Hoemke, L.; Mohlberg, H.; Schleicher, A.; Amunts, K. and Zilles, K. (2008): Stereotaxic probabilistic maps of the magnocellular cell groups in human basal forebrain. NeuroImage, 42(3): 1127–1141

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