HORMONAL PROFILES OF BLOOD PLASMA ASSOCIATED ESTRUS SYNCHRONIZATION USING DIFFERENT CONCENTRATION OF MAP AND ECG IN BARKI EWES

Authors

1 Animal Reproduction Research Institute (ARRI), Agriculture Research Center (ARC), Egypt

2 Faculty of Agriculture, Al-Azhar University, Egypt

3 Faculty of Agriculture, Assiut University, Egypt

Abstract

The current study aimed to characterize the plateau patterns of plasma progesterone in the peripheral circulation during the application of vaginal sponge impregnated with different concentrations of medroxyprogesterone acetate (MAP) and co-treated without and with different doses of equine chorionic gonadotropin (eCG) and its effects on some items of reproductive performance. Determination the onset surges (time and level) of LH and estradiol-17β were also aimed in the study as a necessary pinpoint to the time of ovulation after MAP sponge-eCG treatment. Forty Barki ewes were randomly allocated into 2 main groups (A & B) of equal numbers (n=20/group). Groups A and B were treated with vaginal sponges containing 25 and 50 mg MAP, respectively. On day of sponge's withdrawal, ewes of each main group were randomly reassigned according to eCG dose into three subgroups (1, 2 and 3). Groups A1 and B1 (n=6 each group), served as control, without eCG injection, while groups A2 and B2 (n=7/subgroup) were injected at sponge removal with 300 IU/eCG, whereas, groups A3 and B3 (n=7/subgroup) were injected with 500 IU/eCG. Blood samples were taken immediately before sponge insertion and after the placement subsequently every 2 days and continued to the end of sponge application to studying the plateau pattern of progesterone in the peripheral circulation. Nine hours after sponge removal to 74h latter, blood samples were also taken every 3h and 6h for determination LH and estradiol-17β surges, respectively. The time to onset estrus (h), estrus duration, pregnancy and lambing rates (%) were recorded as reproductive performance measuring related to the study. The started level of plasma progesterone in group A increased gradually with level reaching to its maximum level on days 6 and 8 of sponge application and then rapidly decreases reaching to minimum level on day of sponge removal. On contrast, the patterns of plasma progesterone changes in group B were forcefully increased but with slightly high level from the beginning reaching its highest concentration on day 6 and day 8, then decline gradually to reach lower level on the day of sponge withdraw. The mean time from sponge withdraw to LH and estradiol-17β surges were significantly shorter (P<0.05) in-group A than B and significantly longer (P<0.01) in group A1B1 than other eCG treated groups. Neither MAP nor eCG have significant effects on LH concentration, while, eCG have positive effects on the concentration of estradiol-17β. Generally, the hormonal plateau patterns of group A were associated with acceleration the time to onset estrus with high pregnancy rate. In conclusion, the protocols used for estrus synchronization in sheep are associated with different plateau patterns of circulating reproductive hormones and subsequently affect reproductive performance.
 

Keywords

Full Text

Sheep, Estrus synchronization, Reproductive performance, LH, Progesterone, Estradiol

References

Abecia, J.A.; Forcada, F. and Gonzalez-Bulnes, A. (2011): Pharmaceutical Control of Reproduction in Sheep and Goats. Vet. Clin. Food Anim. Prac., 27: 67–79.
Adib, A.; Freret, S.; Touze, J.; Lomet, D.; Lardic, L.; Chesneau, D.; Estienne, A.; Papillier, P.; Monniaux, D. and Rubio, M. (2014): Progesterone improves the maturation of male-induced preovulatory follicles in anoestrous ewes. Reproduction, (148), 403–416.
Ainsworth, L. and Downey, B.R. (1986): A Controlled Internal Drug-Release Dispenser Containing Progesterone for Control of the Estrous Cycle of Ewes. Theriogenology, 26 [6], 847-857.
Altıncekic, Ş.O.; Koyuncu, M.; Tuncel, E. and Kaymakcı, M. (2011): Kıvırcık Koyunlarında Uremenin Mevsime Bağlılığıve Östrus Boyunca Östradiol-17β ve Progesteron Hormonu Duzeylerinin Değişimi. Hayvansal Üretim., 52(2): 10-19.
Alvarez, L.E.M.; Ceron, J.H.; Padilla, E.G.; Marın G.P. and Valencia, J. (2007): Serum LH peak and ovulation following synchronized estrus in goats. Small Ruminant Research. (69): 124–128.
Amer, H.A. and Hazzaa, A.M. (2009): The effect of different progesterone protocols on the reproductive efficiency of ewes during the non-breeding season. Veterinarski Arhiv. 79 (1): 19-30.
Ashwag, E.M. and Mohamed Nour, M.S. (2015): chronological relationships between Estrus onset, time of LH surge and Ovulation time in does synchronized Withchronogest or CIDR. International Journal of Technical Research and Applications e-ISSN: 2320-8163.
Ashwag, E.M.; Mohamed Nour, M.S. and Ghurashi, M.A.H. (2015): Chronological relationships between oestrus onset, time of LH surge and ovulation time in does synchronized using fluorogestone with different doses of eCG. Res. Opin. Anim. Vet. Sci., 5(4): 183-188.
Ataman, M.B.; Akoz, M. and Akman, O. (2006): Induction of synchronizedoestrus in akkaraman cross-bred ewes during breeding and anestrus seasons: the use of short-term and long-term progesterone treatments, Revue Méd. Vét. 157(5): 257-260.
Barrett, D.M.; Bartlewski, P.M.; Batista-Arteaga, M.; Symington, A. and Rawlings, N.C. (2004): Ultrasound and endocrine evaluation of the ovarian response to a single dose of 500 IU of eCG following a 12-day treatment with progestagen-releasing intravaginal sponges in the breeding and nonbreeding seasons in ewes. Theriogenology; 61, 311–327.
Bartlewski, P.M.; Beard, A.P. and Rawlings, N.C. (1999): The relationship between vaginal mucous impedance and serum concentrations of estradiol and progesterone throughout the sheep estrous cycle. Theriogenology 51, 813-827.
Crosby, T.F.; Boland, M.P. and Gordon, I. (1991): Effect of progestagen treatments on the incidence of estrus and pregnancy rates in ewes. Anim. Reprod. Sci. 24:109-118.
Cumming, I.A.; Buckmaster, J.M.; Blockley, M.A.; Goding, J.R.; Winfield, C.G. and Baxter, R.W. (1973): Constancy of interval between luteinizing hormone release and ovulation in the ewe. Biol. Reprod. 9, 24–29.
D’Souza, K.N. (2013): Effects of a Gonadotropin Mixture on Reproductive Success in Progesterone-Treated Non-Lactating Anestrous Ewes. Master of Science in Reproductive Physiology: UMI Number: 1524600, USA.
Day, M.L. and Geary, T.W. (2005): Handbook of Estrous Synchronization. The Ohio State University, Western Region Publication No. 14.
Dogan, I. and Nur, Z. (2006): Different estrous induction methods during the nonbreeding season in Kivircik ewes. Vet. Med.; 51(4): 133-138.
El-Wakil, Salwa, I.; Shemeis, A.R.; Ahmed, A.M. and Abdallah, O.Y. (2008): Genetic and phenotypic relationships involving body weight, degree of maturity and measurer of gain rate of Barki sheep without having recourse to fitting growth curves. J. Agric. Sci. Mansoura Univ., 33: 4835-4848.
Evans, N.P.; Dahl, G.E.; Padmanabhan, V.; Thun, L.A. and Karsch, F.J. (1997): Estradiol requirements for induction and maintenance of the gonadotropin-releasing hormone surge: implications for neuroendocrine processing of the estradiol signal. Endocrinology; 138:5408–5414.
Fleisch, A.; Werne, S.; Heckendorn, F.; Hartnack, S.; Piechotta, M.; Bollwein, H.; Thun, R. and Janett, F. (2012): Comparison of 6-day progestagen treatment with Chronogest® CR and Eazi-breed CIDR- G intravaginal inserts for estrus synchronization in cyclic ewes. Small Ruminant Research 107, 141–146.
Godfrey, R.W.; Collins, J.R.; Hensley, E.L. and Wheaton, J.E. (1998): Estrus synchronization and artificial insemination of hair sheep ewes in the tropics. Theriogenology, 51: 985-997.
Gonzalez-Bulnes, A.; Souza, C.J.; Campbell, B.K. and Baird, D.T. (2004): Systemic and intraovarian effects of dominant follicles on ovine follicular growth. Anim. Reprod. Sci. 84, 107–119.
Gonzalez-Bulnes, A.; Veiga-Lopez, A.; Garcia, P.; Garcia-Garcia, R.M.; Ariznavarreta, C.; Sanchez, M.A.; Tresguerres, J.A.F.; Cocero, M.J. and Flores, J.M. (2005): Effects of progestagens and prostaglandin analogues on ovarian function and embryo viability in sheep. Theriogenology 63, 2523–2534.
Greyling, J.P.C. and Van der Nest, M. (2000): Synchronization of estrous in goats: dose effect of progestagen. Small Rumin Res.; 36: 201-207.
Greyling, J.P.C.; Erasmus, J.A.; Taylor, G.J.; Van der Merwe, S. (1997): Synchronization of estrus in sheep using progestagen and inseminating with chilled semen during the breeding season. Small Rumin. Res.; 26: 137-143.
Gungor, O.; Ozyurtlu, N.; Pancarci, S.M.; Kaya, M.; Zonturlu, A.K.; Oral, H.; Cetin, Y. and Polat, B. (2009): Estrous synchronization with used CIDR-G devices in ewes during non-breeding sea-son. Kafkas Univ. Vet. Fak. Derg. 15, 779-783.
Habibizad, J.; Riasi, A.; Kohram, H. and Rahmani, H.R. (2015): Effect of feeding greater amounts of dietary energy for ashort-term with or without eCG injection on reproductive performance, serum metabolites and hormones in ewes. Animal Reproduction Science, Volume 160, pages: 82-89.
Hamra, A.H.; Massri, Y.G.; Marcek, J.M. and Wheaton, J.E. (1986): Plasma progesterone levels in ewes treated with progesterone-controlled internal drug-release dispensers, implants and sponges. Anim Reprod Sci; 11: 187-194.
Haresign, W. (1985): Comparison of the rate of decline in plasma progesterone concentrations at a natural and progesterone-synchronized oestrus and its effect on tonic LH secretion in the ewe. J.Rprod.Fert.75, 231-236.
Husein, M.Q. and Kridi, R.T. (2002): Reproductive responses of Awassi ewes treated with either naturally occurring progesterone or synthetic progestagen. Asian-Australian J. Anim. Sci. 9, 1257-1262.
Husein, M.Q. and Ababneh, M.M. (2007): A new strategy for superior reproductive performance of ewes bred out-of-season utilizing progestagen supplement perior to withdrawal of intra vaginal pessaries. Arab Organization for Agricultural Development. Jordan, 176:199.
Husein, M.Q.; Bailey, M.T.; Ababneh, M.M.; Romano, J.E.; Crabo, B.G. and Wheaton, J.E. (1998): Transcervical artificial insemination of ewes out-of season using frozen-thawed semen Effect of equine chorionic gonadotropin on pregnancy rate. Theriogenology, 49: 997-1005.
Jarquin, S.; Roldan, A.; Zarco, L.; Berruecos, J. and Valencia, J. (2014): Effect of stage of the estrous cycle at the time of initial exposure to rams on the ovarian activity of Pelibuey ewes. Czech J. Anim. Sci., 59(11): 504–510.
Karasch, F.J.; Bowen, J.M.; Caraty, A.; Evans, N.P. and Moenter, S.M. (1997): Gonadotropin-releasing hormone requirements for ovulation. Biol. Reprod. 56, 303-309.
Karsch, F.J.; Legan, S.J.; Ryan, K.D. and Foster, D.L. (1980): Importance of estradiol and progesterone in regulating LH secretion and estrous behavior during the sheep estrous cycle. Biol. Reprod. 23:404-413.
Karsch, F.J.; Moenter, S.M. and Caraty, A. (1992): The neuroendocrine signal for ovulation. Animal Reproduction Science 28 329-341.
Kasikci, G.; Cirit, U.; Gunduz, M.C.; Bacinoglu, S. and Sabuncu, A. (2011): Effects of halving intravaginal sponges and eCG dose on estrus response and fertility in Tahirova ewes during the breeding season. Turk. J. Vet. Anim. Sci. 35(3): 193-199.
Kaya, M.; Yildiz, S. and Guven, B. (2005): The determination of plasma progesterone, oestradiol- 17β and luteinizing hormone levels during oestrous period of Tuj ewe-lambs following oestrus induction. Revue Méd. Vét., 156, 12, 628-632.
Kaya, S.; Kacar, C.; Kaya, D. and Aslan, S. (2013): The effectiveness of supplemental administration of progesterone with GnRH, hCG and PGF2_ on the fertility of Tuj sheep during the non-breeding season. Small Ruminant Research 113; 365–370.
Keisler, D.H. and Buckrell, B.C. (1997): Breeding strategies. In: Youngquist R.S. (ed.): Current Therapy in Large Animal Theriogenology. WB Saunders and Co., Philadelphia. 603–611.
Kennedy, D. (2012): Sheep Reproduction Basics and Conception Rates. Order no. 12-037 Agdex 433/30.
Kumar, D. and Naqvi, S.M.K. (2014): Effect of time and depth of insemination on fertility of Bharat Merino sheep inseminated trans-cervical with frozen-thawed semen. Journal of Animal Science and Technology, 56:8.
Kusina, N.T.; Tarwirei, F.; Hamudikuwanda, H.; Agumba, G.; Mukwena, J. (2000): A comparison of the effects of progesterone sponges and ear implants, PGF2alpha, and their combination on efficacy of estrus synchronization and fertility of Mashona goat does. Theriogenology.53: 1567–1580.
Lahoz, B.; Folch, J.; Sanchez, P. and Alabart, J.L. (2013): Characterization of the preovulatory LH surge in Rasa Aragonesa sheep or allele carriers FecXR (ROA®) (ROA®). ITEA, 109 (4), 415-428.
Martemucci, G. and D’Alessandro, A.G. (2011): Synchronization of oestrus and ovulation by short time combined FGA, PGF2, GnRH, eCG treatments for natural service or AI fixed-time. Animal Reproduction Science 123;32–39.
Menchaca, A. and Rubianes, E. (2004): New treatments associated with timed artificial insemination in small ruminants. ReprodFertil Develop, 16, 403-413.
Moakhar, H.K.; Kohram, H.; Shahneh, A.Z. and Saberifar, T. (2012): Ovarian response and pregnancy rate following different doses of eCGtreatment in Chall ewes. Small Ruminant Research;102 , 63– 67.
Mobarak, M.S.; McDonnell, H. and Gordon, I. (1984): The use of progesterone impregnated sponges in intact cyclic ewes bred by natural and artificial insemination. In: Research Report 1982-1983, Faculty of General Agriculture, University College, Dublin; 101-108.
Moenter, S.M.; Caraty, A. and Karsch, F.J. (1990): The estradiol-induced surge of gonadotropin-releasing hormone in the ewe. Endocrinology; 127: 1375–1384.
Mohammed, KM.; Farag, BF.; Daghash, HS. And Azab, MM. (2016): Effect of estrus synchronization protocols on the reproductive performance of Barki sheep.  Assuit Vet. Med. J. vol. 62; No. 151, 22-35.
Mondal, M.; Rajkhowa, C. and Prakash, B.S. (2006): Relationship of plasma estradiol-17b, total estrogen and progesterone to estrous behavior in mithun (Bosfrontalis) cows. Horm Behav 49, 626–633.
Moradi Kor, N.; Khanghah, K.M. and Ali Veisi, A. (2013): Follicular Fluid Concentrations of Biochemical Metabolites and Trace Minerals in Relation to Ovarian Follicle Size in Dairy Cows. Annual Review and Research in Biology 3(4): 397-404.
Munoz-Gutierrez, M.; Blache, D.; Martin, G.B. and Scaramuzzi, R.J. (2002): Folliculogenesis and ovarian expression of mRNA encodingaromatase in anoestrous sheep after 5 days of glucose orglucosamine infusion or supplementary lupin feeding. Reproduction; 124, 721–731.
Munro, R.K. (1987): Concentrations of plasma progesterone in cows after treatment with 3 types of progesterone pessaries. Aust. Vet. J.; 64: 283-284.
Najafi, G.h. and Cedden, F. (2015): Ovarian hormones in Ghezel ewes treated with pregnant mare serum gonadotropin (PMSG) during the breeding season. I.J.A.L.S., Volume (8); 139-144, Issue (2).
Noor Hashida, Syafnir, H. and Meriksa, S. (2013): Time of PMSG administration: Effect on progesterone and estradiol concentration in synchronized ewes. Biomedical Research., 24 (1): 7-12.
Pearce, D.T. and Robinson, T.J. (1985): Plasma progesterone concentrations, ovarian and endocrinological responses and sperm transport in ewes with synchronized oestrus. J. Reprod. Fert. 75, 49-62.
Pierson, J.T.; Baldassarre, H.; Keefer, C.L. and Downey, B.R. (2001): Seasonal variation in preovulatory events associated with synchronization of estrus in dwarf goats. Theriogenology. 56: 759–769.
Rutigliano, H.M. (2010): Using Single Chain Gonadotropins to Enhance Fertility in Sheep. This is a doctoral of philosophy in Animal Biology, university of California, UMI Number: 3427407.
Salamon, S. and Maxwell, W.M.C. (1995): Frozen storage of ram semen. Causes of low fertility after cervical insemination and methods of improvement. Animal Reproduction Science, 38: 1–36.
Salleh, S.M.; Yaakub, H. and Panandam, J.M. (2014): Progesterone and Luteinising Hormone Profile of Saanen Crossbred Goats Synchronised with CIDR and PMSG. Mal. J. Anim. Sci. 17(1): 95-102.
Sareminejad, P.; Tabatabaei, S.; Mamouei, M.; Mirzadeh, K. and Boujarpour, M. (2014): The Effects of Short and Long Term Medroxy Progesterone Acetate (MAP) Sponge Treatments on Reproductive Performance during the Non-Breeding Season of Arabian Ewes. Iranian Journal of Applied Animal Sci.; 4(4), 747-751.
Satterfield, M.C. (2004): Evaluation of the effect of progesterone cidr devices on circulating levels of progesterone in cyclic ewes. Master of science, Texas A&M University.
Scaramuzzi, R.J.; Downing, J.A.; Campbell, B.K. and Cognie, Y. (1988): Control of fertility and fecundity of sheep by means of hormonal manipulation. Aust. J. Biol. Sci. 41: 37-45.
Senger, P.L. (2005): Pathways to pregnancy and Parturation. 2nd revised edition, Current Conceptions, Inc., Pullman.
Simonetti, L.; Blanco, M.R. and Gardoan, J.C. (2000): Estrus synchronization in ewes treated with sponges impregnated with different doses of medroxyprogesterone acetate. Small Ruminant Research 38: 243-247.
Ungerfeld, R. (2003): Reproductive responses of anestrous ewes to the introduction of rams. Doctoral thesis. ISSN 1401-6257.
Ustuner, B.; Gunay, U.; Nur, U. and Ustuner, U. (2007): Effects of long and short-term progestogens treatment combined with PMSG on oestrus synchronization and fertility in Awassi ewes during the breeding season. Journal of ActaVeterinaria Brno. 76:391-397.
Van Cleeff, J.; Karsch, F.J. and Padmanabhan, V. (1998): Characterization of endocrine events during the periestrous period in sheep after estrous synchronization with controlled internal drug release (CIDR) device. Domest Anim Endocrinol; 15:23–34.
Vilarinoa, M.; Rubianes, E.; van Lier, E. and Menchaca, A. (2010): Serum progesterone concentrations, follicular development and time of ovulation using a new progesterone releasing device (DICO®) in sheep. Small Ruminant Research; 91, 219–224.
Vinoles, C.; Forsberg, M.; Banchero, G. and Rubianes, E. (2001): Effect of long-term and short-term progestagen treatment on follicular development and pregnancy rate in cyclic ewes. Theriogenology, 55, 993-1004.
Wheaton, J.E.; Carlson, K.M.; Windels, H.F. and Johnston, L.J. (1993): CIDR a new progesterone-releasing intravaginal device for induction of estrus and cycle control in sheep and goats. Anim. Reprod. Sci. 33, 127–141.
Yavuzer, U. (2005): The possibilities of twice yearly lambing of Awassisheep ewes without using hormones in an organic animal production system. Turk. J. Vet. Anim. Sci. 29: 27-30.
Yildiz, S.; Uzun, M.; Kaya, M.; Ucar, O. and Genesiz, O. (2004): Effects of rams and luteal or follicular phase ewes on preovu-latory LH surge characteristics in ewes. Turk. J. Vet. Anim. Sci. 28, 669-673.
Younes, M. (2008): A comparison of ovarian function in juvenile and adult ewes using in vitro culture and proteomics. Aberystwyth: Aberystwythuniversity; 302P.

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