Assiut University, Faculty of Veterinary MedicineAssiut Veterinary Medical Journal1012-597334.26819960101HISTOLOGICAL CHANGES IN BUFFALO ENDOMETRIUM DURING ESTROUS CYCLE11218365710.21608/avmj.1996.183657ENGALILA A.EL-RAFEYA.S.S.ABDOONJournal Article19951113<strong>Endometrial </strong><strong>specimens </strong><strong>were </strong><strong>obtained </strong><strong>from </strong><strong>normally </strong><strong>cyclic </strong><strong>buffalo </strong><strong>at </strong><strong>known </strong><strong>stages </strong><strong>of </strong><strong>their </strong><strong>estrous </strong><strong>period </strong><strong>to show </strong><strong>precise </strong><strong>characteristic changes</strong><strong>. </strong><br /> <strong>with </strong><strong>These </strong><strong>tissues </strong><strong>w</strong><strong>e</strong><strong>re </strong><strong>embedded </strong><strong>in </strong><strong>paraffin</strong><strong>, </strong><strong>sectioned </strong><strong>an</strong><strong>d stained </strong><strong>hematoxyli</strong><strong>n </strong><strong>and </strong><strong>eos</strong>in. The following histological ch<strong>anges </strong><strong>were </strong><strong>observed</strong><strong>: </strong><strong>During </strong><strong>estrous</strong><strong>, </strong><strong>the </strong><strong>stroma </strong><strong>w</strong><strong>a</strong><strong>s </strong><strong>highly </strong><strong>oedematous </strong><strong>with </strong><strong>highly </strong><strong>congest</strong><strong>e</strong><strong>d </strong><strong>blood </strong><strong>capillaries </strong><strong>and </strong><strong>few </strong><strong>migratory </strong><strong>cells </strong><strong>directed </strong><strong>toward </strong><strong>the </strong><strong>lumen</strong><strong>. </strong><strong>The </strong><strong>endometrial </strong><strong>gla</strong><strong>n</strong><strong>ds </strong><strong>showed </strong><strong>prominent and </strong><strong>regularly </strong><strong>distributed </strong><strong>vacuolation</strong><strong>. </strong><strong>These </strong><strong>vacuoles </strong><strong>displace </strong><strong>the </strong><strong>nuclei </strong><strong>upward </strong><strong>to </strong><strong>create </strong><strong>a </strong><strong>pattern </strong><strong>of </strong><br /> pseudostratification. During met-estrous: The stroma was denser with a marked decrease in oedematous fluid and highly infiltrated lymphocyte and mast cells. Most of the vacuoles of the endometrial glands have discharged its secretion, and a homogenous acidophilic secretion reached its peak and accumulated in the lumen. During di-estrous: The stroma looked like a solid sheet of closely packed stromal cells. Some stromal cells showed signs of transformation into predecidual cells. The glandular epithelium showed an orderly row of nuclei with homogenous acidophilic cytoplasm above them. During pro-estrous, the stroma divided into outer condensed non functional layer and inner loosed layer with few migratory cells. The endometrial glands lined with inactive, low columnar darkly stained cells.https://avmj.journals.ekb.eg/article_183657_95b2078eb6d5561bbfc52f7d6cf22756.pdfAssiut University, Faculty of Veterinary MedicineAssiut Veterinary Medical Journal1012-597334.26819960101CLONING AND SEQUENCING OF GENOME SEGMENT 10 OF AFRICAN HORSESICKNESS VIRUS TYPE 9222918365810.21608/avmj.1996.183658ENA.H.HUSSEINS. MORCHATTMELISSAW.W.LAEGREIDJournal Article19951111African horsesickness virus, Type 9 Plum Island isolate, (AHSV, T9 PI) <strong>wa</strong><strong>s </strong><strong>p < /strong><strong>ropagated </strong><strong>on </strong><strong>VERO </strong>cells till reaching 70<em>% </em>CPE. The double stranded (ds) RNA genome was extracted using the Phenol Chloroform Method,<br /> <strong> </strong><br /> <em>CLONING </em><em>AND </em><em>SEQUENCING </em><em>OF </em><em>GENOME </em><em>OF </em><em>AHS </em><em>VIRUS </em><br /> <em>(</em><em>FAVALORO </em><em>et </em><em>al</em><em>, </em><em>19</em><em>8</em><em>0</em><em>)</em><em>, </em><em>C</em>DNA of S10 was obtained by applying the <strong>Reverse </strong><strong>Transcryptase </strong><strong>(</strong><strong>RT</strong><strong>) </strong><strong>reaction</strong><strong>. </strong><strong>The </strong><strong>exponential </strong><strong>amplification </strong><strong>of </strong><strong>S10 </strong><strong>cDNA </strong><strong>from </strong><strong>the </strong><strong>dsRNA </strong><strong>genome </strong><strong>was </strong><strong>achieved </strong><strong>through </strong><strong>performing </strong><strong>the </strong><strong>Polymerase </strong><strong>Chain </strong><strong>Reaction </strong><strong>(</strong><strong>PCR</strong><strong>) </strong><strong>u</strong><strong>s</strong><strong>ing </strong><strong>2 </strong><strong>primers </strong><strong>designed </strong><strong>in accordance </strong>with the published sequence of the cognate gene. Ligation of S10 to the PCR TM11 ve<strong>ctor </strong><strong>as </strong><strong>well </strong><strong>as </strong><strong>Transformation </strong><strong>o</strong>f the "One Shot" competent cells (NV, F) with Such a p < strong>lasmid </strong><strong>was </strong><strong>carried </strong><strong>out </strong><strong>following </strong><strong>the </strong><strong>instructions </strong><strong>of </strong><strong>the </strong><strong>TA </strong><strong>cloning </strong><strong>Manual </strong><strong>(</strong><strong>Invitrogen</strong><strong>)</strong><strong>. </strong><strong>The </strong><strong>assertiveness </strong><strong>of </strong><strong>th</strong>e proper ligation <strong>and </strong><strong>transformation </strong><strong>w</strong><strong>as </strong><strong>a</strong><strong>ss</strong><strong>e</strong><strong>ssed </strong>by applying the minilysate preparation protocol and checked up in 1.2% agarose gel electrophoresis for the right size of S10 gene <em>( </em>~700 bp) For obtaining an adequate amount of plasmid <strong>BNA </strong><strong>needed </strong><strong>for </strong><strong>se</strong><strong>quence </strong><strong>analysis </strong><strong>of </strong><strong>S10</strong><strong>, </strong><strong>PEG </strong><strong>plasmid </strong><strong>preparation </strong>protocol was performed. The nucleotide sequence of S10 w<strong>as </strong><strong>determined </strong><strong>by </strong>using version 2.0-DNA Sequencing Kit based on the strategy of the Dideoxy <strong>Termination </strong><strong>Reactio</strong>n <em>(</em><em>SANGER </em><em><span style="text-decoration: underline;">et </span></em><em><span style="text-decoration: underline;">al </span></em><em>1977</em><em>)</em>. This study proved that there is a high degree of homolo<span style="text-decoration: underline;">gy </span>in the nucleotide Sequence of AHSV-T9 PI, S10 and the published sequence of the cognate gene so that it could be of diagnostic value. It is also a preliminary step for further investigations for the role of, s10 gene in AHS virus replication as well as its pathogenesis. This <strong>work </strong><strong>wa</strong><strong>s </strong><strong>a</strong><strong>ccom</strong>plished in Plum Island laboratories (USA) and the associated authors shared in.https://avmj.journals.ekb.eg/article_183658_e8ad18bc872f5c1e48e304a002a82b8c.pdfAssiut University, Faculty of Veterinary MedicineAssiut Veterinary Medical Journal1012-597334.26819960101BACTERIAL CAUSES AND ANTIBIOGRAM OF MASTITIS IN DAIRY FRIESIAN COWS AT ASSIUT GOVERNORATE222918366210.21608/avmj.1996.183662ENA.M.SAYEDJournal Article19960128<strong>This </strong><strong>study </strong><strong>was </strong><strong>carried </strong>out on 36 dairy <strong>friesian </strong><strong>cows </strong><strong>suffered </strong><strong>from </strong><strong>asti</strong><strong>t</strong><strong>is </strong><strong>at </strong><strong>A</strong><strong>s</strong>siut Governorate. E.coli, Streptococcal uberis, Kelbsiella <strong>pneumoniae </strong><strong>an</strong><strong>d </strong><strong>C</strong><strong>.</strong><strong>bovis </strong><strong>formed </strong><strong>the main </strong><strong>bacterial </strong><strong>causes </strong><strong>of </strong><strong>mastitis</strong><strong>. </strong><strong>They </strong>were isolated and identified from 41.6%, 22.2, 22<em>.</em><em>2</em>% and 13.9% of the total <strong>infections </strong><strong>respectiv</strong>ely. E.coli (54.5%) and Streptococcal uberis <em>(</em>27.2<em>% </em>were the most prevalent pathogens during the first month of la<strong>ctation</strong><strong>, </strong><strong>while </strong><strong>kellbsiella </strong><strong>pneumoniae </strong><strong><em>(</em></strong><strong>62</strong><strong>.</strong><strong>5</strong><strong>%</strong><strong>)</strong><strong>, </strong><strong>and </strong><strong>Corybnebacterium </strong><strong>bovis </strong><strong><em>(</em></strong><strong>66</strong><strong>.</strong><strong>6</strong><strong>%</strong><strong>) </strong><strong>were </strong><strong>the </strong><strong>predominant </strong><strong>causes </strong><strong>fr</strong>om the period of 31-90 days and more than 90 days <strong>respectively</strong><strong>. </strong><strong>The </strong><strong>largest </strong><strong>percentage </strong><strong>of </strong><strong>intramammary </strong><strong>infection </strong><strong>(</strong><strong>IM</strong><strong>I</strong><strong>) </strong><strong>were </strong><br /> detected during the summer season (83.3%), and early stage of lactation (61.1%). Antibiogram study revealed that gentamycin, and kanamycin had highly inhibitory effect on isolated strains of E.coli, Streptococcal uberis, Kelbsiella and pneumoniae C.bovis. https://avmj.journals.ekb.eg/article_183662_bf073d5c504744a87eaa35fd6e450b5c.pdfAssiut University, Faculty of Veterinary MedicineAssiut Veterinary Medical Journal1012-597334.26819960101MICROBIAL ASPECTS OF LOCALLY MANUFACTURED MAYONNAISE303618366610.21608/avmj.1996.183666ENA.A.BAHOUTA.NASSARE.A.SALEHJournal Article19950522https://avmj.journals.ekb.eg/article_183666_8cb4655d21e388e371629b8866d32c83.pdfAssiut University, Faculty of Veterinary MedicineAssiut Veterinary Medical Journal1012-597334.26819960101A RAPID TWO WEEKS-EVALUATION OF VITAMIN C & B-COMPLEX, AND SODIUM CHLORIDE FOR HEAT-STRESSED BROILERS374718390610.21608/avmj.1996.183906ENA.N.SAYEDH.SHOEIBJournal Article19951223<strong>S<em>UMMARY </em></strong><strong>Two </strong><strong>e</strong><strong>x</strong><strong>periments </strong><strong>were </strong><strong>conducted </strong><strong>to </strong><strong>determine </strong><strong>t</strong>he efficacy of Vit. C, Vit.B complex and sodium chloride treatments for heat-stressed Arbor acres <strong>chicken</strong><strong>s</strong><strong>. </strong><strong>T</strong><strong>he </strong><strong>birds </strong><strong>were </strong><strong>given </strong><strong>access </strong><strong>to </strong><strong>water </strong><strong>and </strong><strong>commer</strong>cial diet containing 21.4% protein, 2<em>97</em>3 (Kcal<em>/</em>Kg diet) metabolizable energy, 2.9% <strong>fat </strong><strong>an</strong>d 3.1% fibre. At the age of 28 days, the birds in <strong>the </strong><strong>two </strong><strong>experiments </strong>were subject<strong>ed </strong><strong>to </strong><strong>the </strong><strong>different </strong><strong>treatment</strong>s. In the first experiment, the birds <strong>were </strong><strong>subjected </strong><strong>to </strong><strong>drinking </strong><strong>water </strong><strong>supplemented </strong><strong>w</strong>ith vit.C (0.5g/litre), vit B complex (1g/litre) <strong>and </strong><strong>sodium </strong>chloride <em>(</em>0.5g<em>/</em>litre<strong>) </strong><strong>and </strong><strong>maintained </strong><strong>at </strong><strong>a </strong><strong>constant </strong><strong>temperatur</strong>e of 38°C at day time and 35°C at night. In comparison with control, body weight was improved by 10%, 8.8% with vit.C and sodium chloride treatments (p < 0.05) respectively. The trial then repeated on large scale (field trial) in which the birds were subjected to the same drinking <strong>water </strong><strong>supplementation </strong><strong>of </strong>vit.B-complex and sodium chloride, while vit.C was added to the diet (200 mg<em>/</em>kg diet). The birds were exposed to an ambient temperature ranging from 35-39°C. Body weight was improved by 6.8% with vit.C (p < 0.05) and 5.5% with sodium chloride. Vitamins and sodium chlroride reduced mortality related to heat-stress. It could be concluded that Vit. C supplementation either in drinking water or in the diet and sodium chloride are beneficial for heat-stressed broilers.https://avmj.journals.ekb.eg/article_183906_ad4e0a85e97cfe71e62fe1341e24071f.pdfAssiut University, Faculty of Veterinary MedicineAssiut Veterinary Medical Journal1012-597334.26819960101SEPTIC ARTHRITIS IN DONKEYS485518390710.21608/avmj.1996.183907ENM.M.ALIM.M. ABDEL-HAFEEZJournal Article19951220Bacterial examination of the synovial effusion of 26 donkey joints (15 carpal, 10 fetlock and one pastern) revealed isolation of staph. sp as a prodominant microorganism in septic arthritis. <em>Staph </em><em>aure</em><em>u</em><em>s </em>represented (14.28%) and <em>Sta</em><em>ph</em><em>. </em><em>ep < /em><em>i</em><em>dermedis </em>was (61.9%). <em>Corynebacterium </em><em>e</em><em>qui</em><em>, </em><em>Corynebacteri</em><em>u</em><em>m </em><em>pyogen</em><em>es</em><em>, </em><em>E</em><em>nterobac</em><em>ter </em><em>a</em><em>erogene</em>s and Shigella sp. were also isolated. The antibiogram of the isolated microorganisms revealed that bactrim is the antibiotic of choice followed by rifampicin, flumequine and chloramphenicol. All strains showed resistance to polymyxin.</em>https://avmj.journals.ekb.eg/article_183907_bed1f0cb1e4236d9b6d3379f99ca48fe.pdfAssiut University, Faculty of Veterinary MedicineAssiut Veterinary Medical Journal1012-597334.26819960101EFFECT OF EXTRACTED CRUDE VENOM FROM JELLY FISH EUTONINA INDICANS ON LIVER FUNCTION IN MALE MICE566718390810.21608/avmj.1996.183908ENSOHEIR A.ABD-EL-REHIMNAGWA M.EL-SAWIH.M.ABOUL-DAHABJournal Article19951128<strong>S<em>UMMARY </em></strong>A sublethal dose of crude venom extracted from the Jelly fish <em>Eutonina </em><strong><em>indicans </em></strong><strong>w</strong><strong>a</strong><strong>s </strong><strong>injected </strong><strong>intraperitoneally </strong><strong>t</strong>o mice (1.78 m<em>g</em><em>/</em>20 gm b.w.). The percentages of blood serum ( A1, A2, B, V, ) globulins and albumin were determined. Also, the levels of liver proteins, <span style="text-decoration: underline;">gly</span>cogen, total lipid, TBARS, <strong>transaminases </strong><strong>(</strong><strong>S</strong><strong>. </strong><strong>GOT </strong><strong>& </strong><strong>S</strong><strong>. </strong><strong>GPT</strong><strong>) </strong><strong>alkaline </strong><strong>a</strong><strong>n</strong><strong>d </strong><strong>a</strong>cid phosphatases were <strong>determined</strong><strong>. </strong><strong>After </strong><strong>24 </strong><strong>hours </strong><strong>o</strong><strong>f </strong><strong>treatmen</strong><strong>t</strong><strong>, </strong><strong>th</strong><strong>e </strong><strong>crude </strong><strong>venom </strong><strong>induced </strong><br /> <em>EFFECT </em><em>OF </em><em>JEL</em><em>LY </em><em>FISH </em><em>VENOM </em><em>O</em>N <em>LIVER </em><em>FUNCTIO</em>N<br /> insignificant (P >0.05) changes of Q1, A2, B, v, globulins and albumiin percentages, on the other hand, this ex<strong>tracted </strong><strong>crude </strong><strong>venom induced </strong><strong>a </strong>significant (P<0.001) increase in the liver total proteins and glycogen levels, while the levels of lipid, T<strong>BARS</strong><strong>, </strong><strong>transaminases </strong><strong>(</strong><strong>S</strong><strong>. </strong><strong>GOT </strong><strong><em>& </em></strong>S. GPT) alkaline and acid phosphatases activities showed in-significant (P>0.05) changes. <strong>This </strong><strong>extracted </strong><strong>crude </strong><strong>venom </strong><strong>w</strong><strong>as </strong><strong>p < /strong><strong>erforme</strong>d on Nicolet apparatus cod 710 FT-IR spectrometer which predict that the structure of the venom may be aliphatic compound. The biological effect of this <strong>extracted </strong><strong>venom </strong><strong>may </strong><strong>be </strong>attributed to the direct effect of crude venom bradykinin or direct effect through the activation of prostaglandin synthesis.</strong>https://avmj.journals.ekb.eg/article_183908_cbbc3ca7747d97ee3189a028c51a0755.pdfAssiut University, Faculty of Veterinary MedicineAssiut Veterinary Medical Journal1012-597334.26819960101LEVELS OF SOME HEAVY METALS IN FISH CAUGHT FROM RIVER NILE AT ASSIUT GOVERNORATE, EGYPT688418391010.21608/avmj.1996.183910ENM.ABD EL-NASSERAnimal Health Research Institute, AssiutA.A.HAABANDept. of Forensic Medicine and Toxicology,
Faculty of Veterinary Medicine, Assiut University,SFHAM, M.ALYAnimal Health Research Institute, AssiutMAnalM. SA YEDAnimal Health Research Institute, AssiutJournal Article19960108https://avmj.journals.ekb.eg/article_183910_86a6b90342a1281730166adfc1e0979e.pdfAssiut University, Faculty of Veterinary MedicineAssiut Veterinary Medical Journal1012-597334.26819960101LEAD, COPPER, MERCURY AND CADMIUM LEVELS IN RIVER NILE WATERS AT SOME ASSIUT REGIONS, EGYPT.859418391210.21608/avmj.1996.183912ENM.ABD EL-NASSERA.A.SHAABANSEHAM, M.ALYMANAL M.SAYEDJournal Article19960108<strong>SUMMARY </strong>The increased use of heavy metals like lead, copper, mercury and cadmium in industry and agriculture, and thier subsequent in<strong>trusion </strong><strong>in </strong><strong>indeterminate </strong><strong>amounts </strong>into the environment has caused ecological and biological changes. In our study 50 <strong>water </strong><strong>samples </strong><strong>w</strong><strong>e</strong><strong>re </strong><strong>collected </strong><strong>from </strong><strong>Abu</strong><strong>-</strong><strong>Tig</strong><strong>, </strong><strong>Assiut </strong><strong>Manquabad</strong><strong>, </strong><strong>Bany</strong><strong>-</strong><strong>Qurra </strong><strong>and </strong><strong>Dairut</strong><strong>, </strong><strong>at </strong><strong>winter </strong><strong>and </strong><strong>summ</strong>er 1992<em>/</em>1993. Water samples were subjected to analysis for the presence of lead, copper, <strong>mercury </strong><strong>and </strong><strong>cadmium</strong><strong>. </strong><strong>Our </strong><strong>results r</strong>evealed that lead concentrations in water of the different ex<strong>amined </strong><strong>areas </strong><strong>w</strong>ere 1.940, 0.810, <strong>0</strong><strong>.</strong><strong>810</strong><strong>, </strong><strong>0</strong><strong>.</strong><strong>3</strong><strong>!</strong><strong>4 </strong><strong>and </strong><strong>0</strong><strong>.</strong><strong>790 </strong><strong>ppm </strong><strong>in </strong><strong>winter </strong><strong>an</strong>d 0.220, 0.270, 0.313, 0.222 and 0.200 ppm in summer at Abu-T<span style="text-decoration: underline;">ig</span>, Assiut, Manqabad, Bany-Qurra and Dairut respectively. Abu-T<span style="text-decoration: underline;">i</span>g and Assiut areas registered the h<span style="text-decoration: underline;">i</span>ghest concentration in winter and Manqabad and Assiut in summer. The examined water samples were above the permissible limit of WHO (0.10 ppm) or United States Environmental Protection Agency (US EPA) which is 0.05 ppm. The highest concentration of copper in water samples collected from Manqabad and Bany-Qurra were 0.42 and 0.088 ppm in <strong>winter </strong><strong>an</strong>d 0.522 and 0.39 p < strong>pm <strong>in </strong><strong>summer</strong><strong>. </strong><strong>The </strong><strong>examined </strong><strong>water </strong><strong>samples </strong><strong>showed </strong>values less than the permissible limits adopted by US EAP (1.0 ppm). A <strong>concentrati</strong>on of 18.700 and 12.400 ppb were the hig<strong>hest </strong><strong>concentrations </strong><strong>of </strong><strong>mercury </strong><strong>in </strong><strong>water </strong><strong>s</strong>amples collected <strong>from </strong><strong>Assiut </strong><strong>and </strong><strong>Bany</strong><strong>-</strong><strong>Qurra </strong><strong>in </strong><strong>winter </strong><strong>an</strong>d 24.800 and 15.600 ppb in Bany-Qurra and Dairut in <strong>summer</strong><strong>. </strong><strong>The </strong><strong>examined water </strong><strong>samples </strong><strong>showed </strong><strong>values </strong><strong>more </strong><strong>than </strong><strong>the </strong>permissible limits adopted by US EP<em>A </em>(2 ppb). The <strong><span style="text-decoration: underline;">hig</span></strong><strong>hest </strong><strong>concentration </strong><strong>of </strong><strong>cadmium </strong><strong>in </strong><strong>wat</strong><strong>er </strong><strong>at </strong><strong>B</strong>any-Qurra and Manqabad (0.009 <strong>and </strong><strong>0</strong><strong>.</strong><strong>007 </strong><strong>ppm</strong><strong>) </strong><strong>in </strong><strong>winter </strong><strong>and </strong><strong>Dairut </strong><strong>and </strong><strong>Manqabad </strong>(0.012 and 0.015 ppm) in <strong>summer</strong><strong>. </strong><strong>Water </strong><strong>samples </strong><strong>showed </strong><strong>cadmium </strong><strong>values </strong><strong>less </strong><strong>tha</strong><strong>n </strong><strong>the </strong><strong>permi</strong><strong>s</strong><strong>sible </strong><strong>limit </strong>(0.01 ppm) excep < strong>t <strong>wa</strong><strong>te</strong><strong>r </strong><strong>sampl</strong>es collected from M<strong>anqabad </strong><strong>and </strong><strong>Dairut </strong><strong>in </strong><strong>summer </strong><strong>which </strong><strong>reached </strong><strong>t</strong>o 0.015 and 0.012 ppm respectively.https://avmj.journals.ekb.eg/article_183912_e40b300953c796b7723d983bb729c0f2.pdfAssiut University, Faculty of Veterinary MedicineAssiut Veterinary Medical Journal1012-597334.26819960101CADMIUM, LEAD, NICKEL, COPPER, MANGANESE AND FLUORINE LEVELS IN RIVER NILE FISH9510218391310.21608/avmj.1996.183913ENA.SH.SEDDEKD.A.SALEMNAGWA, M.EL-SAWIZ.M.ZAKYJournal Article19960124Five Nile fish species <em>Orechromis </em><em>niloticus</em><em>, </em><em>Clarias </em><em>lazera</em><em>, </em><em>Labeo </em><em>niloticus</em><em>, </em><strong><em>Synodontis </em></strong><strong><em>an</em></strong><strong><em>d </em></strong><strong><em>Bagr</em></strong><strong><em>u</em></strong><strong><em>s </em></strong><strong><em>bayad </em></strong><strong>w</strong><strong>e</strong><strong>re </strong><strong>collected </strong><strong>from fish </strong><strong>sources</strong><strong>. </strong><strong>Twenty </strong><br /> 95<br /> <em>LEVELS </em><em>OF </em>S<em>OME </em><em>ELEMENTS </em><em>IN </em><em>FISH </em><br /> samples from each were weighed and muscles were d<span style="text-decoration: underline;">i</span>gested, analyzed and calculated for cadmium, lead, fluorine, nickel, copper and manganese. The results revealed that the averages of the previously mentioned elements were 0.26, 0.33, 0.37, 0.33 and 0.39 ppm, 0.32, 0.45, 0.86, 0.64 and 0.12 ppm, 0.38, 0.20, 0.33, 0.12 and 0.16 ppm, 1.20, 2.48, 0.75, 0.83 and 0.84 ppm, 4.22, 5.55, 2.16, 3.78 and 1.68 ppm and 2.8, 3.2, 2.2, 2.9 and 1.2 ppm, respectively. The results indicate an increase of cadmium, lead levels and a slight increase in copper and nickel in fish muscles. The results are discussed with other previous reports and its hazardous health effects.https://avmj.journals.ekb.eg/article_183913_8a964bc8ff2ef581f3d49145ec0a7939.pdfAssiut University, Faculty of Veterinary MedicineAssiut Veterinary Medical Journal1012-597334.26819960101REPRODUCTIVE PERFORMANCE IN RATS AND EWES TREATED WITH PYRETHROID (ECTOMIN)10312318391510.21608/avmj.1996.183915ENFATMA, M.SALEMGALILA, A.EL-RAFEYH.A.EL-MANSOURYAMANY, E.YOUSSEFJournal Article19951230The present study aimed to study the reproductive effect of Ectomin as pyrethroid insecticide in albino <strong>female </strong><strong>and </strong><strong>male </strong><strong>rats </strong><strong>as </strong><strong>a </strong><strong>model </strong><strong>of </strong><strong>experimental </strong><strong>animals </strong><strong>and </strong><strong>ewes </strong><strong>as </strong><strong>a </strong><strong>model </strong><strong>of </strong><strong>farm </strong><strong>animal</strong><strong>. </strong><strong>Twenty </strong><strong>five </strong><strong>male </strong><strong>rat</strong><strong>s </strong><strong>were </strong><strong>used </strong><strong>for </strong><strong>male </strong><strong>fertility</strong><strong>. </strong><strong>They </strong><strong>were </strong><strong>given </strong><strong>Ectomin </strong>(31 mg<em>/</em>kg .b.wt) <strong>for </strong><strong>60 </strong><strong>d</strong><strong>ays</strong><strong>. </strong><strong>Animals </strong><strong>w</strong><strong>e</strong><strong>re </strong><strong>sacrificed </strong><strong>every </strong><strong>15 </strong><strong>days</strong><strong>. </strong><strong>Ectomin </strong><strong>resulted </strong><strong>in </strong><strong>a </strong>significant decrease in weight of testis, <strong>seminal </strong><strong>vesicles </strong><strong>prostate </strong>gland. It also cause a significant decrease in all parameters of male fertility (progressive motility, live sperms and sperm concentration) in addition to significant increase in sperm abnormalities. Histological studies revealed that Ectomin induce testicular dysfunction by inducing clear signs of degenerative changes of the spermatogenic cells, detachment of necrotic cells, desquamation of normal intact spermatocyte and in addition to clear destruction of interstitial tissue. Forty mature albino <strong>female </strong><strong>r</strong><strong>ats </strong><strong>were </strong><strong>u</strong><strong>sed </strong><strong>f</strong>or teratological examination. They were given Ectomin on day 6-15 of gestation (the period of organogenesis). 1-The morphological examination revealed the presence of implantation sites, resorbed foetuses and the living foetuses showed decreased body weight. 2-The visceral examination revealed the presence of cardiomegaly, dilatation of the renal pelvis and hypoplasia of the lung. 3- The skeletal examination revealed the presence of incomplete ossification of the skull, large open fontanelle and absence of sternbrae. Ten ewes were used for hormonal profiles. Ewes were sprayed with Ectomin (1<em>/</em>500 in D.W) during the 1 st day of heat and second spraying was applied 15 days later. The present result revealed that spraying of Barki ewes by Ectomin had no <strong>impairment </strong><strong>effect </strong>neither on the sequencing of estrous cycle nor its length. A <strong>significant </strong><strong>decrease </strong><strong>of </strong><strong>P4 </strong><strong>(</strong><strong>progesterone</strong><strong>) was </strong><strong>obse</strong>rved on day 9 of estrous cycle (starting of peak level) in the <span style="text-decoration: underline;">2nd </span>and 3 <span style="text-decoration: underline;">rd </span>estrous cycle, and on day 12 of the three estrous cycle followed spraying. On the other hand neither the <strong>pattern of </strong><strong>gonadotrophins </strong><strong>(</strong><strong>FSH </strong><strong>& </strong><strong>LH</strong><strong>) </strong><strong>nor </strong><strong>their </strong><strong>levels </strong><strong>was </strong><strong>affected </strong><strong>by </strong><strong>Ectomin</strong><strong>. </strong>https://avmj.journals.ekb.eg/article_183915_43ea709c9b37bde3f4d51471918aca62.pdfAssiut University, Faculty of Veterinary MedicineAssiut Veterinary Medical Journal1012-597334.26819960101LACTATE DEHYDROGENASE AND PHOSPI-IATASES ENZYMES LEVELS IN OVIDUCAL FLUSHING FLUID OF EGYPTIAN BUFFALO-COWS WITH ACTIVE AND INACTIVE OVARIES12413718391810.21608/avmj.1996.183918ENGA.MEGHEDAJMA.MEKIMMANWARJournal Article20211107https://avmj.journals.ekb.eg/article_183918_62390fe7f720eecf9b110a3b86f0b672.pdfAssiut University, Faculty of Veterinary MedicineAssiut Veterinary Medical Journal1012-597334.26819960101STUDIES ON THE EFEECT OF CRYPTOSPORIDIUM ON THE IMMUNE RESPONSE TO DUCK VIRUS HEPATITIS VACCINE13814818391910.21608/avmj.1996.183919ENN.ABED EL-WANISAFAF HAMDYAMINNADIA M.HASSANNAGWA E.AHMEDJournal Article19960106For studying the effect of cryptosporidiosis on the immune response of ducklings vaccinated with Duck Virus Hepatitis vaccine, 120 one day old, white pekin duckling were divided into 5 groups each of 24 ducklings. Two groups were infected at, one day old and vaccinated at either 1 and 7 days. The other 3 groups were kept as controls (one for infection, one for vaccination and the other as blank). It was found that duckling that were infected and vaccinated showed low level of antibody titer compared to the control group. The prepatent period was 5 days in the 3 experimentally infected groups, whereas the patent varied where it was 23, 24 and 21 days in group I, II and III respectively.https://avmj.journals.ekb.eg/article_183919_674136f0368f378bf35ca37b88480439.pdfAssiut University, Faculty of Veterinary MedicineAssiut Veterinary Medical Journal1012-597334.26819960101EPIDEMIOLOGICAL PROPERTIES OF PUS-FORMING BACTERIA14915718392010.21608/avmj.1996.183920ENA.M.ABEL-FATTAHJournal Article19951205The epidemiological properties of Nocardia species as a soil inhabitant organism was observed, among 65 soil samples yielded Nocardia species in a percentage (12.54%) While it was isolated in (23.33%) from mastitic milk originated from cases of bovine mastitis. In addition, some geophilic species of fungi such as Aapergillus, Penicillum, Mucor and Candida were also isolated from soils, as well as the frequently detected bacteria in cases of mastitis as Staphlococci, Coliforms and Candida.https://avmj.journals.ekb.eg/article_183920_44d3d5c5e21b1b5da3d2a7d50e32b81e.pdf